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Literature summary for 4.3.1.24 extracted from

  • Goldson-Barnaby, A.; Scaman, C.H.
    Purification and characterization of phenylalanine ammonia lyase from Trichosporon cutaneum (2013), Enzyme Res., 2013, 670702.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
L-tyrosine pH 8.5, 37°C, Vmax: 0.82 microM/min, trial 3 Cutaneotrichosporon cutaneum
2
-
L-tyrosine pH 8.5, 37°C, Vmax: 1 microM/min, trial 2 Cutaneotrichosporon cutaneum
2.4
-
L-tyrosine pH 8.5, 37°C, Vmax: 0.2 microM/min, trial 1 Cutaneotrichosporon cutaneum
4.2
-
L-phenylalanine pH 8.5, 37°C, Vmax: 7.2 microM/min, trial 3 Cutaneotrichosporon cutaneum
5
-
L-phenylalanine pH 8.5, 37°C, Vmax: 1.3 microM/min, trial 1 Cutaneotrichosporon cutaneum
5.7
-
L-phenylalanine pH 8.5, 37°C, Vmax: 4.7 microM/min, trial 2 Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
SDS-PAGE Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography give a purification fold of 50 with 20% yield Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
Cutaneotrichosporon cutaneum (E)-cinnamate + NH3
-
?
L-tyrosine
-
Cutaneotrichosporon cutaneum ?
-
?

Synonyms

Synonyms Comment Organism
TcPAL
-
Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
Cutaneotrichosporon cutaneum

pH Range

pH Minimum pH Maximum Comment Organism
8 8.5
-
Cutaneotrichosporon cutaneum