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Literature summary for 4.3.1.24 extracted from

  • Lovelock, S.; Turner, N.
    Bacterial Anabaena variabilis phenylalanine ammonia lyase: A biocatalyst with broad substrate specificity (2014), Bioorg. Med. Chem., 22, 5555-5557.
    View publication on PubMed
Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Trichormus variabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 (2E)-3-phenylprop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.07
-
 (2E)-3-(3-fluorophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.12
-
 (2E)-3-(4-fluorophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.23
-
 (2S)-2-amino-3-(4-nitrophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.29
-
 (2S)-2-amino-3-phenylpropanoic acid pH 8.3, 30°C Trichormus variabilis
0.38
-
 (2E)-3-(pyridin-4-yl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.39
-
 (2E)-3-(pyridin-3-yl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.48
-
 (2S)-2-amino-3-(pyridin-4-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.56
-
 (2S)-2-amino-3-(4-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.66
-
 (2E)-3-(4-nitrophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.75
-
 (2S)-2-amino-3-(3-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.05
-
 (2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid pH 8.3, 30°C Trichormus variabilis
1.11
-
 (2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.51
-
 (2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
3.3
-
 (2E)-3-(3-hydroxyphenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
3.39
-
 (2S)-2-amino-3-(pyridin-3-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis

Organism

Organism UniProt Comment Textmining
Trichormus variabilis Q3M5Z3
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Trichormus variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-amino-3-(3-fluorophenyl)propanoic acid
-
 Trichormus variabilis (2E)-3-(3-fluorophenyl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid
-
 Trichormus variabilis (2E)-3-(3-hydroxyphenyl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-(4-fluorophenyl)propanoic acid
-
 Trichormus variabilis (2E)-3-(4-fluorophenyl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-(4-nitrophenyl)propanoic acid
-
 Trichormus variabilis (2E)-3-(4-nitrophenyl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-(pyridin-3-yl)propanoic acid
-
 Trichormus variabilis (2E)-3-(pyridin-3-yl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-(pyridin-4-yl)propanoic acid
-
 Trichormus variabilis (2E)-3-(pyridin-4-yl)prop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-phenylpropanoic acid
-
 Trichormus variabilis (2E)-3-phenylprop-2-enoic acid + NH3
-
 r
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid
-
 Trichormus variabilis (2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid + NH3
-
 r

Synonyms

Synonyms Comment Organism
AvPAL
-
 Trichormus variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 (2E)-3-phenylprop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.08
-
 (2E)-3-(3-fluorophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.09
-
 (2E)-3-(4-fluorophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.14
-
 (2E)-3-(pyridin-3-yl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.49
-
 (2S)-2-amino-3-phenylpropanoic acid pH 8.3, 30°C Trichormus variabilis
0.61
-
 (2E)-3-(3-hydroxyphenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
0.61
-
 (2S)-2-amino-3-(pyridin-4-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.82
-
 (2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid pH 8.3, 30°C Trichormus variabilis
0.89
-
 (2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.91
-
 (2S)-2-amino-3-(pyridin-3-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.06
-
 (2S)-2-amino-3-(4-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.06
-
 (2E)-3-(pyridin-4-yl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
1.08
-
 (2S)-2-amino-3-(4-nitrophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.1
-
 (2S)-2-amino-3-(3-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
3.14
-
 (2E)-3-[4-(trifluoromethyl)phenyl]prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis
3.81
-
 (2E)-3-(4-nitrophenyl)prop-2-enoic acid pH 8.3, 30°C Trichormus variabilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.27
-
 (2S)-2-amino-3-(pyridin-3-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis
0.78
-
 (2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid pH 8.3, 30°C Trichormus variabilis
0.8
-
 (2S)-2-amino-3-(3-hydroxyphenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.27
-
 (2S)-2-amino-3-(pyridin-4-yl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.47
-
 (2S)-2-amino-3-(3-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
1.69
-
 (2S)-2-amino-3-phenylpropanoic acid pH 8.3, 30°C Trichormus variabilis
1.89
-
 (2S)-2-amino-3-(4-fluorophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis
4.7
-
 (2S)-2-amino-3-(4-nitrophenyl)propanoic acid pH 8.3, 30°C Trichormus variabilis