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Literature summary for 4.3.1.24 extracted from

  • Hsieh, L.S.; Ma, G.J.; Yang, C.C.; Lee, P.D.
    Cloning, expression, site-directed mutagenesis and immunolocalization of phenylalanine ammonia-lyase in Bambusa oldhamii (2010), Phytochemistry, 71, 1999-2009.
    View publication on PubMed
Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bambusa oldhamii

Protein Variants

Protein Variants Comment Organism
F133H decrease in ration kcat/KM value Bambusa oldhamii
F134H decrease in ratio kcat/KM value Bambusa oldhamii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 L-Tyr pH 8.5, 37°C Bambusa oldhamii
0.23
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii
0.333
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii
0.335
-
 L-Tyr mutant F134H, pH 8.5, 37°C Bambusa oldhamii
0.993
-
 L-Tyr wild-type, pH 8.5, 37°C Bambusa oldhamii
2.07
-
 L-Phe pH 8.5, 37°C Bambusa oldhamii
2.288
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii
4.22
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75711
-
 4 * 75711, calculated Bambusa oldhamii
76000
-
 4 * 76950, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii
76000
-
 4 * 76975, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii
76950
-
 4 * 76950, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii
76975
-
 4 * 76975, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii
290000
-
 gel filtration Bambusa oldhamii
320000
-
 gel filtration Bambusa oldhamii
330000
-
 gel filtration Bambusa oldhamii

Organism

Organism UniProt Comment Textmining
Bambusa oldhamii C0LL35 isoform PAL2
-
Bambusa oldhamii D5KS97 isoform PAL4
-
Bambusa oldhamii Q6ST23
-
-

Source Tissue

Source Tissue Comment Organism Textmining
shell
-
 Bambusa oldhamii
-
shoot in shoot and branch shoot, mainly localized to sclerenchymal cells Bambusa oldhamii
-
shoot in shoot and branch shoot, mainly loclaized to sclerenchymal cells Bambusa oldhamii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
 Bambusa oldhamii (E)-cinnamate + NH3
-
 ?
L-Tyr poor substrate Bambusa oldhamii (2E)-4-hydroxycinnamate + NH3
-
 ?
L-Tyr
-
 Bambusa oldhamii p-coumarate + NH3
-
 ?
additional information no substrate: L-Tyr Bambusa oldhamii ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 75711, calculated Bambusa oldhamii
tetramer 4 * 76950, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii
tetramer 4 * 76975, calculated, 4 * 76000, SDS-PAGE Bambusa oldhamii

Synonyms

Synonyms Comment Organism
PAL2
-
 Bambusa oldhamii
PAL4
-
 Bambusa oldhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bambusa oldhamii
60
-
-
 Bambusa oldhamii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 L-Tyr wild-type, pH 8.5, 37°C Bambusa oldhamii
0.1
-
 L-Tyr mutant F134H, pH 8.5, 37°C Bambusa oldhamii
0.54
-
 L-Tyr pH 8.5, 37°C Bambusa oldhamii
7.8
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii
16.3
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii
16.32
-
 L-Phe pH 8.5, 37°C Bambusa oldhamii
19.2
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii
21.3
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bambusa oldhamii
9
-
-
 Bambusa oldhamii

pI Value

Organism Comment pI Value Maximum pI Value
Bambusa oldhamii calculated
-
 5.9

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.028
-
 L-Tyr wild-type, pH 8.5, 37°C Bambusa oldhamii
0.18
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii
0.31
-
 L-Tyr mutant F134H, pH 8.5, 37°C Bambusa oldhamii
5.56
-
 L-Tyr pH 8.5, 37°C Bambusa oldhamii
7.88
-
 L-Phe pH 8.5, 37°C Bambusa oldhamii
8.4
-
 L-Phe mutant F134H, pH 8.5, 37°C Bambusa oldhamii
63.9
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii
70.8
-
 L-Phe wild-type, pH 8.5, 37°C Bambusa oldhamii