Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Bambusa oldhamii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.01 | - |
L-phenylalanine | recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C | Bambusa oldhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76950 | - |
subunit, calculated from amino acid sequence | Bambusa oldhamii |
80000 | - |
4 * 80000, subunit, SDS-PAGE | Bambusa oldhamii |
323000 | - |
gel filtration | Bambusa oldhamii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bambusa oldhamii | Q6ST23 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni2+-chelating column chromatography | Bambusa oldhamii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Bambusa oldhamii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Bambusa oldhamii | (E)-cinnamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, subunit, SDS-PAGE | Bambusa oldhamii |
Synonyms | Comment | Organism |
---|---|---|
EC 4.3.1.5 | formerly | Bambusa oldhamii |
PAL1 | isoform | Bambusa oldhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bambusa oldhamii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Bambusa oldhamii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.11 | - |
L-phenylalanine | recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C | Bambusa oldhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bambusa oldhamii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | - |
Bambusa oldhamii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-phenylalanine | recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C | Bambusa oldhamii |