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Literature summary for 4.3.1.24 extracted from
- Strategies to maximize the encapsulation efficiency of phenylalanine ammonia lyase in microcapsules (2008), Int. J. Pharm., 356, 61-68.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | activity PAL encapsulated in cellulose nitrate microcapsules is only 23% of the activity of PAL in Tris buffer due to its incomplete encapsulation, optimzation of encapsulation method and efficiency, method, PAL activity free in the aqueous core of the microcapsules is 85.7% of the total activity in the homogenate of the microcapsules, while the activity of PAL bound to the membrane of the microcapsules is 14.3% of the total activity in the homogenate of the microcapsules, overview | Rhodotorula glutinis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
4 * 80000, SDS-PAGE | Rhodotorula glutinis |
| 320000 | - |
gel filtration | Rhodotorula glutinis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | Rhodotorula glutinis | - |
(E)-cinnamate + NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| further 2fold purification of the commercial preparation by gel filtration | Rhodotorula glutinis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Rhodotorula glutinis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative enzyme activity and encapsulation efficiency in microcapsules, overview | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 80000, SDS-PAGE | Rhodotorula glutinis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAL | - |
Rhodotorula glutinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rhodotorula glutinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Rhodotorula glutinis |
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Release 2023.1 (January 2023)
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