Any feedback?
Literature summary for 4.3.1.24 extracted from
- Catalytic properties of Rhodotorula aurantiaca KM-1 phenylalanine ammonia-lyase (2009), Appl. Biochem. Microbiol., 45, 17-21.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca |  |
| 2-mercaptoethanol | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis | |
| ascorbic acid | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca | |
| ascorbic acid | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis | |
| EDTA | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Buckleyzyma aurantiaca | |
| EDTA | activation by 2-mercaptoethanol, EDTA, and ascorbic acid. The effects of EDTA and ascorbic acid are additive | Rhodotorula glutinis | |
General Stability
| General Stability | Organism |
|---|---|
| enzyme stabilization by 20% glycerol, 2-mercaptoethanol at 10 mM, 5 mM EDTA, and 2% ascorbic acid or 5 mM EDTA + 2% ascorbic acid | Buckleyzyma aurantiaca |
| glycerol stabilizes the enzyme | Rhodotorula glutinis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-phenylalanine | competitive inhibition | Buckleyzyma aurantiaca | |
| D-phenylalanine | competitive inhibition | Rhodotorula glutinis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Buckleyzyma aurantiaca | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Rhodotorula glutinis | |
| 0.25 | - |
L-phenylalanine | - |
Rhodotorula glutinis | |
| 1.33 | 1.75 | L-phenylalanine | in presence or absence of D-phenylalanine | Buckleyzyma aurantiaca | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | Buckleyzyma aurantiaca | - |
(E)-cinnamate + NH3 | - |
? | |
| L-phenylalanine | Rhodotorula glutinis | - |
(E)-cinnamate + NH3 | - |
? | |
| L-phenylalanine | Buckleyzyma aurantiaca KM-1 | - |
(E)-cinnamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Buckleyzyma aurantiaca | - |
- |
- |
| Buckleyzyma aurantiaca KM-1 | - |
- |
- |
| Rhodotorula glutinis | Q2VMT1 | fragment | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.07 | 2.5 | - |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | - |
Buckleyzyma aurantiaca | (E)-cinnamate + NH3 | - |
? | |
| L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
| L-phenylalanine | - |
Buckleyzyma aurantiaca KM-1 | (E)-cinnamate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-phenylalanine ammonia-lyase | - |
Buckleyzyma aurantiaca |
| L-phenylalanine ammonia-lyase | - |
Rhodotorula glutinis |
| PAL | - |
Buckleyzyma aurantiaca |
| PAL | - |
Rhodotorula glutinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Buckleyzyma aurantiaca |
| 30 | - |
assay at | Rhodotorula glutinis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
15 min, loss of 50% activity | Buckleyzyma aurantiaca |
| 57 | - |
15 min, loss of 50% activity | Rhodotorula glutinis |
| 65 | - |
10 min, loss of activity | Buckleyzyma aurantiaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
assay at | Buckleyzyma aurantiaca |
| 8.9 | - |
assay at | Rhodotorula glutinis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.55 | - |
most stable at | Buckleyzyma aurantiaca |
| 6.55 | - |
most stable at | Rhodotorula glutinis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Buckleyzyma aurantiaca | |
| additional information | - |
additional information | inhibition kinetics | Rhodotorula glutinis | |
| 3.3 | - |
D-phenylalanine | - |
Rhodotorula glutinis | |
| 3.38 | - |
D-phenylalanine | - |
Buckleyzyma aurantiaca | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
