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Literature summary for 4.3.1.24 extracted from

  • Watts, K.T.; Mijts, B.N.; Lee, P.C.; Manning, A.J.; Schmidt-Dannert, C.
    Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family (2006), Chem. Biol., 13, 1317-1326.
    View publication on PubMed
Search for more literature for 4.3.1.24

Protein Variants

Protein Variants Comment Organism
F144H decrease of activity with L-phenylalanine, increase of activity with L-tyrosine Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0428
-
 L-phenylalanine wild-type, pH 9.0, 37°C Arabidopsis thaliana
0.532
-
 L-tyrosine mutant F144H, pH 9.0, 37°C Arabidopsis thaliana
1.049
-
 L-tyrosine wild-type, pH 9.0, 37°C Arabidopsis thaliana
1.386
-
 L-phenylalanine mutant F144H, pH 9.0, 37°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P35510
-
-

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine = trans-cinnamate + NH3 E1cb mechanism Arabidopsis thaliana
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Arabidopsis thaliana (E)-cinnamate + NH3
-
 ?
L-tyrosine
-
 Arabidopsis thaliana p-coumarate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PAL
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.084
-
 L-tyrosine wild-type, pH 9.0, 37°C Arabidopsis thaliana
0.75
-
 L-tyrosine mutant F144H, pH 9.0, 37°C Arabidopsis thaliana
47.3
-
 L-phenylalanine mutant F144H, pH 9.0, 37°C Arabidopsis thaliana
65
-
 L-phenylalanine wild-type, pH 9.0, 37°C Arabidopsis thaliana
115.8
-
 L-phenylalanine wild-type, pH 9.0, 37°C Arabidopsis thaliana