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Literature summary for 4.3.1.24 extracted from

  • El-Shora, H.M.
    Properties of phenylalanine ammonia-lyase from marrow cotyledons (2002), Plant Sci., 162, 1-7.
No PubMed abstract available
Search for more literature for 4.3.1.24

Activating Compound

Activating Compound Comment Organism Structure
NaCl maximal activity at 200 mM, inhibition above Cucurbita pepo

Inhibitors

Inhibitors Comment Organism Structure
ADP 5 mM, approx. 50% inhibition Cucurbita pepo
AMP 5 mM, approx. 40% inhibition Cucurbita pepo
ATP 5 mM, approx. 80% inhibition Cucurbita pepo
beta-chloroethyltrimethylammonium 10 mM, 60% inhibition of PAL activity in cotyledons Cucurbita pepo
Ca2+ 6 mM, 40% inhibition Cucurbita pepo
EDTA 35 mM, complete inactivation Cucurbita pepo
N,N-Dicyclohexylcarbodiimide 10 mM, 82% inhibition Cucurbita pepo
N-acetylimidazol 10 mM, 75% inhibition Cucurbita pepo
o-phenanthroline 20 mM, complete inactivation Cucurbita pepo
Phenylmethylsulphonyl fluoride 10 mM, 59% inhibition Cucurbita pepo

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ approx. 3fold activation at 8 mM Cucurbita pepo
Mn2+ approx. 1.5fold activation at 8 mM Cucurbita pepo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine Cucurbita pepo first enzyme in the phenylpropanoid biosynthetic pathway (E)-cinnamate + NH3
-
 r

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol stabilizes Cucurbita pepo

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
 marrow
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, Sephadex G-100, Q-Sepharose Cucurbita pepo

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Cucurbita pepo
-
seed
-
 Cucurbita pepo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.6
-
-
 Cucurbita pepo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Cucurbita pepo (E)-cinnamate + NH3
-
 ?
L-phenylalanine first enzyme in the phenylpropanoid biosynthetic pathway Cucurbita pepo (E)-cinnamate + NH3
-
 r

Synonyms

Synonyms Comment Organism
PAL
-
 Cucurbita pepo

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5
-
 40% and 90% loss of activity after 1 and 5 days, respectively Cucurbita pepo
5
-
 approx. 30% loss of activity after 5 days in the presence of 30% glycerol Cucurbita pepo