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Literature summary for 4.3.1.24 extracted from
- Purification and characterization of phenylalanine ammonia-lyase from Ustilago maydis (1996), Phytochemistry, 43, 351-357.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-aminoindane-2-phosphonate | - |
Ustilago maydis |  |
| 2-mercaptoethanol | above 25 mM | Ustilago maydis | |
| 8-hydroxyquinoline | - |
Ustilago maydis | |
| Ag+ | - |
Ustilago maydis | |
| Cinnamic acid | trans-cinnamate, moderately; trans-cinnamic acid | Ustilago maydis | |
| CN- | NaCN | Ustilago maydis | |
| Cu2+ | - |
Ustilago maydis | |
| Hg2+ | - |
Ustilago maydis | |
| NaBH4 | - |
Ustilago maydis | |
| p-chloromercuribenzoate | - |
Ustilago maydis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.05 | - |
L-Phe | - |
Ustilago maydis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for metal ion cofactors | Ustilago maydis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
4 * 80000, SDS-PAGE | Ustilago maydis |
| 320000 | - |
non-denaturing PAGE | Ustilago maydis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ustilago maydis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | no glycosylation | Ustilago maydis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ustilago maydis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ustilago maydis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5% loss of activity after 1 week, 40% loss of activity after 1 month | Ustilago maydis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe | - |
Ustilago maydis | (E)-cinnamate + NH3 | - |
? | |
| L-tyrosine | no deamination of L-Tyr | Ustilago maydis | p-coumarate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 80000, SDS-PAGE | Ustilago maydis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Ustilago maydis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.0, 10 min, stable | Ustilago maydis |
| 60 | - |
pH 7.0, 10 min, 30% loss of activity | Ustilago maydis |
| 70 | - |
pH 7.0, 10 min, complete loss of activity | Ustilago maydis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9.2 | - |
Ustilago maydis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9.2 | pH 6.5: 50% of maximal activity, pH 8.8-9.2: optimum | Ustilago maydis |
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