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Literature summary for 4.3.1.24 extracted from

  • Sarma, A.D.; Sreelakshmi, Y.; Sharma, R.
    Differential expression and properties of phenylalanine ammonia-lyase isoforms in tomato leaves (1998), Phytochemistry, 49, 2233-2243.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.121
-
L-Phe enzyme form PAL-II Solanum lycopersicum
0.465
-
L-Phe enzyme form PAL-III Solanum lycopersicum
0.84
-
L-Phe enzyme form PAL-I Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
4 * 80000, SDS-PAGE Solanum lycopersicum
320000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum 3 isoforms, which are differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 isoenzymes: PAL-I, PAL-II and PAL-III Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
Solanum lycopersicum (E)-cinnamate + NH3
-
?
additional information 3 isoforms, which are differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway Solanum lycopersicum ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 80000, SDS-PAGE Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
enzyme form PAL-II Solanum lycopersicum
9.5
-
enzyme form PAL-III Solanum lycopersicum
10.5
-
enzyme form PAL-I Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7: about 50% of maximal activity, pH 10.5: about 70% of maximal activity, enzyme form PAL-II Solanum lycopersicum
7 10.5 pH 7: about 30% of maximal activity, pH 10.5: about 90% of maximal activity, enzyme form PAL-III Solanum lycopersicum
8 11 pH 8: about 50% of maximal activity, pH 11: about 80% of maximal activity, enzyme form PAL-I Solanum lycopersicum