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Literature summary for 4.3.1.23 extracted from

  • Kyndt, J.A.; Meyer, T.E.; Cusanovich, M.A.; Van Beeumen, J.J.
    Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein (2002), FEBS Lett., 512, 240-244.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
L-tyrosine 35°C, pH 8.5 Rhodobacter capsulatus
1.28
-
L-phenylalanine 35°C, pH 9.4 Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine Rhodobacter capsulatus
-
(E)-cinnamate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombiannt TAL Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
Rhodobacter capsulatus (E)-cinnamate + NH3
-
?
L-phenylalanine
-
Rhodobacter capsulatus (E)-cinnamate + NH3
-
r
L-tyrosine
-
Rhodobacter capsulatus p-hydroxycinnamic acid + NH3
-
?

Synonyms

Synonyms Comment Organism
TAL
-
Rhodobacter capsulatus
tyrosine ammonia lyase
-
Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.1
-
L-phenylalanine 35°C, pH 9.4 Rhodobacter capsulatus
27.7
-
L-tyrosine 35°C, pH 8.5 Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
deaminantion of L-tyrosine Rhodobacter capsulatus
9.4
-
deaminantion of L-phenylalanine Rhodobacter capsulatus

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 deamination of L-tyrosine, approx. 14% of maximal activity at pH 6.0, approx. 20% of maximal activity at pH 10.5, deaminantion of L-phenylalanine, approx. 1% of maximal activity at pH 6.0, approx. 75% of maximal activity at pH 10.5 Rhodobacter capsulatus