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Literature summary for 4.3.1.20 extracted from
- Purification and properties of erythro-beta-hydroxyaspartate dehydratase from Micrococcus denitrificans (1965), Biochem. J., 97, 547-554.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Pyridinecarboxylic acid | - |
Paracoccus denitrificans |  |
| EDTA | 0.1 mM almost complete inhibition, activity restored by divalent cations | Paracoccus denitrificans | |
| hydroxylamine | - |
Paracoccus denitrificans | |
| Maleate | competitive | Paracoccus denitrificans | |
| p-chloromercuribenzoate | GSH protects | Paracoccus denitrificans | |
| Semicarbazide | - |
Paracoccus denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value varied with pH, optimum at pH 7.8 | Paracoccus denitrificans | |
| 0.43 | - |
erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
| Mg2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
| Mn2+ | restores activity after EDTA treatment | Paracoccus denitrificans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Erythro-3-hydroxyaspartate | Paracoccus denitrificans | beta-hydroxyaspartate pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Paracoccus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.15 | - |
- |
Paracoccus denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 60 days | Paracoccus denitrificans |
| 2°C, with 0.01M Tris-HCl buffer, pH 8, 1 mM MgCl2 and 0.01 mM pyridoxal 5'-phosphate, 50% loss of activity in 10 days | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Erythro-3-hydroxyaspartate | beta-hydroxyaspartate pathway | Paracoccus denitrificans | ? | - |
? | |
| Erythro-DL-beta-hydroxyaspartate | irreversible, enzyme is highly substrate specific using only the L-isomer | Paracoccus denitrificans | Oxaloacetate + NH3 | - |
? | |
| erythro-L-beta-hydroxyaspartate | - |
Paracoccus denitrificans | oxaloacetate + NH3 | - |
? | |
| additional information | enzyme does not use threo-DL-beta-hydroxyaspartate, erythro and threo-DL-beta-hydroxy-beta methylaspartate, allo D-isomers of serine, threonine and allothreonine, D and L-aspartate, and isomers of tartrate | Paracoccus denitrificans | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Paracoccus denitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Paracoccus denitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | rapid loss of activity below pH 6 and above pH 10 | Paracoccus denitrificans |
| 7.5 | 9.5 | most stable | Paracoccus denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | activates | Paracoccus denitrificans | |
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