Application | Comment | Organism |
---|---|---|
synthesis | starting from simple non-chiral dicarboxylic acids (either fumaric acid or mesaconic acid), vitamin B5 and both diastereoisomers of alpha-methyl-substituted vitamin B5, which are valuable precursors for promising antimicrobials against Plasmodium falciparum and multidrug-resistant Staphylococcus aureus, can be generated in good yields (up to 70%) and excellent enantiopurity (>99% ee). Access to vitamin B5 ((R)-pantothenic acid) and both diastereoisomers of alpha-methyl-substituted vitamin B5 ((R)- and (S)-3-((R)-2,4-dihydroxy-3,3-dimethylbutanamido)-2-methylpropanoic acid) is achieved using a modular three-step biocatalytic cascade involving 3-methylaspartate ammonia lyase (MAL), aspartate-a-decarboxylase (ADC), beta-methylaspartate-alpha-decarboxylase (CrpG) or glutamate decarboxylase (GAD), and pantothenate synthetase (PS) enzymes | Clostridium tetanomorphum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threo-3-methylaspartate | Clostridium tetanomorphum | the enzyme is part of a catabolic pathway for L-glutamate | mesaconate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | Q05514 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | mesaconate + NH3 | - |
r | |
L-threo-3-methylaspartate | the enzyme is part of a catabolic pathway for L-glutamate | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
mesaconate + NH3 | - |
Clostridium tetanomorphum | L-threo-3-methylaspartate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3-methylaspartate ammonia lyase | - |
Clostridium tetanomorphum |
MAL | - |
Clostridium tetanomorphum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of a catabolic pathway for L-glutamate | Clostridium tetanomorphum |