Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridium tetanomorphum |
Protein Variants | Comment | Organism |
---|---|---|
H194A | enhanced diastereoselectivities | Clostridium tetanomorphum |
K331A | enhanced diastereoselectivities | Clostridium tetanomorphum |
Q329A | enhanced diastereoselectivities | Clostridium tetanomorphum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
mesaconic acid | wild-type | Clostridium tetanomorphum | |
1 | - |
L-threo-3-methylaspartic acid | wild-type | Clostridium tetanomorphum | |
1 | - |
L-threo-3-methylaspartic acid | mutant Q329A | Clostridium tetanomorphum | |
1.7 | - |
mesaconic acid | mutant Q329A | Clostridium tetanomorphum | |
1.8 | - |
L-threo-3-methylaspartic acid | mutant H194A | Clostridium tetanomorphum | |
14 | - |
mesaconic acid | mutant H194A | Clostridium tetanomorphum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | Q05514 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threo-3-methylaspartic acid | assay at pH 9.0, 30°C | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
mesaconic acid + NH3 | assay at pH 9.0, 30°C | Clostridium tetanomorphum | L-threo-3-methylaspartate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3-methylaspartate ammonia lyase | - |
Clostridium tetanomorphum |
MAL | - |
Clostridium tetanomorphum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Clostridium tetanomorphum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-threo-3-methylaspartic acid | mutant Q329A | Clostridium tetanomorphum | |
0.25 | - |
mesaconic acid | mutant Q329A | Clostridium tetanomorphum | |
0.55 | - |
L-threo-3-methylaspartic acid | mutant H194A | Clostridium tetanomorphum | |
8.4 | - |
mesaconic acid | mutant H194A | Clostridium tetanomorphum | |
61 | - |
mesaconic acid | wild-type | Clostridium tetanomorphum | |
89 | - |
L-threo-3-methylaspartic acid | wild-type | Clostridium tetanomorphum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Clostridium tetanomorphum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
mesaconic acid | mutant K331A, value below 0.000001 | Clostridium tetanomorphum | |
additional information | - |
L-threo-3-methylaspartic acid | mutant K331A, value below 0.000001 | Clostridium tetanomorphum | |
0.01 | - |
L-threo-3-methylaspartic acid | mutant Q329A | Clostridium tetanomorphum | |
0.15 | - |
mesaconic acid | mutant Q329A | Clostridium tetanomorphum | |
0.31 | - |
L-threo-3-methylaspartic acid | mutant H194A | Clostridium tetanomorphum | |
0.6 | - |
mesaconic acid | mutant H194A | Clostridium tetanomorphum | |
87 | - |
mesaconic acid | wild-type | Clostridium tetanomorphum | |
89 | - |
L-threo-3-methylaspartic acid | wild-type | Clostridium tetanomorphum |