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Literature summary for 4.3.1.2 extracted from
- 3-Methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae (1997), Arch. Microbiol., 168, 457-463.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | enhances activity | Citrobacter freundii |  |
| Dimethylsulfoxide | enhances activity | Morganella morganii | |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae | Citrobacter freundii |
| analysis | a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae | Morganella morganii |
| analysis | a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae | Raoultella planticola |
| analysis | a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae | Citrobacter koseri |
| analysis | a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae | Citrobacter amalonaticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Citrobacter freundii |
| 40000 | - |
2 * 40000, SDS-PAGE | Citrobacter koseri |
| 42000 | - |
2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
| 44000 | - |
2 * 44000, SDS-PAGE | Morganella morganii |
| 45000 | - |
2 * 45000, SDS-PAGE | Raoultella planticola |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threo-3-methylaspartate | Citrobacter freundii | - |
? | - |
? | |
| L-threo-3-methylaspartate | Clostridium tetanomorphum | - |
? | - |
? | |
| L-threo-3-methylaspartate | Morganella morganii | - |
? | - |
? | |
| L-threo-3-methylaspartate | Raoultella planticola | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter koseri | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter amalonaticus | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter amalonaticus YG-1002 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter amalonaticus YG1002 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter freundii YG-0504 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Morganella morganii NCIMB 232 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Raoultella planticola IAM 1133 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter freundii TPU 0011 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter koseri JCM 1659 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Morganella morganii NCIMB 9525 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Citrobacter koseri JCM 1658 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Morganella morganii YG-0601 | - |
? | - |
? | |
| L-threo-3-methylaspartate | Morganella morganii NCIMB 10466 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter amalonaticus | - |
formerly Escherichia coli | - |
| Citrobacter amalonaticus YG-1002 | - |
formerly Escherichia coli | - |
| Citrobacter freundii | - |
- |
- |
| Citrobacter freundii | - |
IFO 13545 | - |
| Citrobacter freundii | - |
IFO 13539 | - |
| Citrobacter freundii | - |
TPU 0011 | - |
| Citrobacter freundii | - |
IFO 13546 | - |
| Citrobacter freundii TPU 0011 | - |
TPU 0011 | - |
| Citrobacter freundii YG-0504 | - |
- |
- |
| Citrobacter koseri | - |
JCM 1659 | - |
| Citrobacter koseri | - |
JCM 1658 | - |
| Citrobacter koseri JCM 1658 | - |
JCM 1658 | - |
| Citrobacter koseri JCM 1659 | - |
JCM 1659 | - |
| Clostridium tetanomorphum | - |
- |
- |
| Morganella morganii | - |
- |
- |
| Morganella morganii | - |
NCTC 1707 | - |
| Morganella morganii | - |
NCTC 2815 | - |
| Morganella morganii | - |
NCIMB 232 | - |
| Morganella morganii | - |
NCTC 10011 | - |
| Morganella morganii | - |
NCIMB 9525 | - |
| Morganella morganii | - |
NCTC 5845 | - |
| Morganella morganii | - |
NCIMB 10466 | - |
| Morganella morganii | - |
IFO 3168 | - |
| Morganella morganii | - |
NCTC 2818 | - |
| Morganella morganii | - |
NCTC 11354 | - |
| Morganella morganii | - |
NCTC 12028 | - |
| Morganella morganii | - |
IFO 3848 | - |
| Morganella morganii | - |
NCTC 10375 | - |
| Morganella morganii | - |
NCTC 7381 | - |
| Morganella morganii NCIMB 10466 | - |
NCIMB 10466 | - |
| Morganella morganii NCIMB 232 | - |
NCIMB 232 | - |
| Morganella morganii NCIMB 9525 | - |
NCIMB 9525 | - |
| Morganella morganii YG-0601 | - |
- |
- |
| Raoultella planticola | - |
IAM 1133 | - |
| Raoultella planticola IAM 1133 | - |
IAM 1133 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions | Citrobacter freundii |
| enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions | Morganella morganii |
| enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions | Raoultella planticola |
| additional information | Citrobacter freundii |
| additional information | Morganella morganii |
| additional information | Raoultella planticola |
| additional information | Citrobacter amalonaticus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| IAM 1133 | Raoultella planticola |
| JCM 1658, JCM 1659 | Citrobacter koseri |
| YG-0504, TPU 0011, IFO 13539, IFO 13545, IFO 13546 | Citrobacter freundii |
| YG-0601, IFO 3168, IFO 3848, NCIMB 232, NCIMB 9525, NCIMB 10466, NCTC 1707, NCTC 2815, NCTC 2818, NCTC 5845, NCTC 7381, NCTC10011, NCTC 10375, NCTC11354, NCTC 12028 | Morganella morganii |
| YG-1002, IFO 13547 | Citrobacter amalonaticus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Citrobacter freundii | |
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Clostridium tetanomorphum | |
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Morganella morganii | |
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Raoultella planticola | |
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Citrobacter koseri | |
| L-threo-3-methylaspartate = mesaconate + NH3 | mechanism | Citrobacter amalonaticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Citrobacter freundii |
| additional information | - |
- |
Clostridium tetanomorphum |
| additional information | - |
- |
Morganella morganii |
| additional information | - |
- |
Raoultella planticola |
| additional information | - |
- |
Citrobacter koseri |
| additional information | - |
- |
Citrobacter amalonaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threo-3-methylaspartate | r | Citrobacter freundii | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Morganella morganii | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Raoultella planticola | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter koseri | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter freundii | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Raoultella planticola | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter koseri | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter amalonaticus YG1002 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus YG1002 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter freundii YG-0504 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter freundii YG-0504 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Morganella morganii NCIMB 232 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii NCIMB 232 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Raoultella planticola IAM 1133 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Raoultella planticola IAM 1133 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter freundii TPU 0011 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter freundii TPU 0011 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter koseri JCM 1659 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter koseri JCM 1659 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Morganella morganii NCIMB 9525 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii NCIMB 9525 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Citrobacter koseri JCM 1658 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter koseri JCM 1658 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Morganella morganii YG-0601 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii YG-0601 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | r | Morganella morganii NCIMB 10466 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii NCIMB 10466 | mesaconate + NH3 | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter freundii | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Morganella morganii | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Raoultella planticola | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter koseri | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter amalonaticus | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter amalonaticus YG-1002 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter amalonaticus YG1002 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter freundii YG-0504 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Morganella morganii NCIMB 232 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Raoultella planticola IAM 1133 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter freundii TPU 0011 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter koseri JCM 1659 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Morganella morganii NCIMB 9525 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Citrobacter koseri JCM 1658 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Morganella morganii YG-0601 | ? | - |
? | |
| L-threo-3-methylaspartate | - |
Morganella morganii NCIMB 10466 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Clostridium tetanomorphum |
| dimer | 2 * 45000, SDS-PAGE | Raoultella planticola |
| dimer | 2 * 44000, SDS-PAGE | Morganella morganii |
| dimer | 2 * 40000, SDS-PAGE | Citrobacter freundii |
| dimer | 2 * 40000, SDS-PAGE | Citrobacter koseri |
| dimer | 2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
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