Application | Comment | Organism |
---|---|---|
synthesis | the mutant L-threonine deaminase G323D/F510L/T344A together with thermostable L-leucine dehydrogenase from Bacillus sphaericus DSM730 and formate dehydrogenase from Candida boidinii constitute a one-pot system for biosynthesis of L-2-aminobutyric acid | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of the mutant enzyme G323D/F510L/T344A in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A14T | the mutant enzyme shows higher thermostability than wild-type enzyme without considerable loss in activity | Escherichia coli |
F510L | mutant enzyme with greater thermostability compared to wild-type enzyme | Escherichia coli |
G323D | mutant enzyme with greater thermostability compared to wild-type enzyme | Escherichia coli |
G323D/F510L/T344A | the half-life of mutant enzyme at 42°C increases from 10 to 210 min, a 20fold increase compared to the wild-type enzyme, and the temperature at which the activity of the enzyme decreases by 50% in 15 min increases from 39 to 53°C | Escherichia coli |
R449C | the mutant enzyme shows higher thermostability than wild-type enzyme without considerable loss in activity | Escherichia coli |
T344A | the mutant enzyme shows higher thermostability than wild-type enzyme without considerable loss in activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 3.5 | L-threonine | pH 7.5, 30°C, mutant enzyme G323D/R449C | Escherichia coli | |
14.5 | - |
L-threonine | pH 7.5, 30°C, wild-type enzyme | Escherichia coli | |
26.2 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A | Escherichia coli | |
26.4 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L | Escherichia coli | |
26.9 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A/R449C | Escherichia coli | |
27.1 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/R449C | Escherichia coli | |
28.3 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme F510L | Escherichia coli | |
30.1 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme A14T/G323D | Escherichia coli | |
32.5 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme A14T | Escherichia coli | |
32.8 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme R449C | Escherichia coli | |
33.3 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme T344A | Escherichia coli | |
33.6 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D | Escherichia coli | |
33.7 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/T344A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine | Escherichia coli | - |
2-oxobutanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P04968 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
68.5 | - |
pH 7.5, 30°C, mutant enzyme A14T | Escherichia coli |
77.4 | - |
pH 7.5, 30°C, mutant enzyme T344A | Escherichia coli |
78.8 | - |
pH 7.5, 30°C, mutant enzyme F510L | Escherichia coli |
89.5 | - |
pH 7.5, 30°C, mutant enzyme R449C | Escherichia coli |
99.4 | - |
pH 7.5, 30°C, mutant enzyme G323D/F510L | Escherichia coli |
99.5 | - |
pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A | Escherichia coli |
99.5 | - |
pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A/R449C | Escherichia coli |
99.5 | - |
pH 7.5, 30°C, mutant enzyme G323D/T344A | Escherichia coli |
100.2 | - |
pH 7.5, 30°C, mutant enzyme G323D/R449C | Escherichia coli |
100.4 | - |
pH 7.5, 30°C, mutant enzyme A14T/G323D | Escherichia coli |
101 | - |
pH 7.5, 30°C, mutant enzyme G323D/F510L/R449C | Escherichia coli |
104.6 | - |
pH 7.5, 30°C, mutant enzyme G323D | Escherichia coli |
108 | - |
pH 7.5, 30°C, wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine | - |
Escherichia coli | 2-oxobutanoate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-TD | - |
Escherichia coli |
L-threonine deaminase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
15 min, 50% loss of activity, wild-type enzyme | Escherichia coli |
41 | - |
15 min, 50% loss of activity, mutant enzyme A14T | Escherichia coli |
41.2 | - |
15 min, 50% loss of activity, mutant enzyme R449C | Escherichia coli |
42 | - |
15 min, 50% loss of activity, mutant enzyme T344A | Escherichia coli |
42 | - |
half-life: 10 min (wild-type enzyme), 210 min (mutant enzyme G323D/F510L/T344A), 14 min (mutant enzyme A14T), 19 min (mutant enzyme T344A), 15 min (mutant enzyme R449C), 57 min (mutant enzyme G323D), 44 min (mutant enzyme F510L), 54 min (mutant enzyme A14T/G323D and mutant enzyme G323D/R449C), 61 min (mutant enzyme G323D/T344A), 201 min (mutant enzyme G323D/F510L), 200 min (mutant enzyme G323D/F510L/R449C), 211 min (mutant enzyme G323D/F510L/T344A/R449C) | Escherichia coli |
44.8 | - |
15 min, 50% loss of activity, mutant enzyme F510L | Escherichia coli |
46 | - |
15 min, 50% loss of activity, mutant enzyme A14T/G323D | Escherichia coli |
46 | - |
15 min, 50% loss of activity, mutant enzyme G323D/R449C | Escherichia coli |
46.2 | - |
15 min, 50% loss of activity, mutant enzyme G323D | Escherichia coli |
47.1 | - |
15 min, 50% loss of activity, mutant enzyme G323D/T344A | Escherichia coli |
52.4 | - |
15 min, 50% loss of activity, mutant enzyme G323D/F510L/R449C | Escherichia coli |
52.5 | - |
15 min, 50% loss of activity, mutant enzyme G323D/F510L | Escherichia coli |
53 | - |
15 min, 50% loss of activity, mutant enzyme G323D/F510L/T344A | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1230 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme F510L | Escherichia coli | |
1256 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme T344A | Escherichia coli | |
1261 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme A14T | Escherichia coli | |
1273 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme R449C | Escherichia coli | |
1283 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L | Escherichia coli | |
1298 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/R449C | Escherichia coli | |
1301 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A/R449C | Escherichia coli | |
1309 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/F510L/T344A | Escherichia coli | |
1312 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/R449C | Escherichia coli | |
1369 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme A14T/G323D | Escherichia coli | |
1394 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D/T344A | Escherichia coli | |
1398 | - |
L-threonine | pH 7.5, 30°C, mutant enzyme G323D | Escherichia coli | |
1414 | - |
L-threonine | pH 7.5, 30°C, wild-type enzyme | Escherichia coli |