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Literature summary for 4.3.1.19 extracted from
- Biochemical characterization of the threonine deaminase (2018), Biochemistry, 57, 6003-6012 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-valine | activator, (0-10 mM) leads to a nearly 50% increase in MsIlvA activity | Mycolicibacterium smegmatis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycolicibacterium smegmatis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K67A | inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme | Mycolicibacterium smegmatis |
| K67Q | inactive mutant enzyme, the mutant enzyme is not yellow, as observed for the wild-type enzyme | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Cycloserine | powerful suicide inactivator that generates a covalent pyridoxal 5'-phosphate-isoxazole complex | Mycolicibacterium smegmatis | |
| L-isoleucine | less than 2% activity remains after addition of 0.5 mM L-isoleucine | Mycolicibacterium smegmatis | |
| L-valine | - |
Mycolicibacterium smegmatis | |
| Mn2+ | 50 mM, 50% loss of activity | Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.66 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 2.86 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 3.01 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 106.3 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50 mM, 1.7fold activation | Mycolicibacterium smegmatis | |
| K+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
| Li+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
| Mg2+ | 50 mM, 1.6fold activation | Mycolicibacterium smegmatis | |
| Na+ | 50 mM, 1.5fold activation | Mycolicibacterium smegmatis | |
| NH4+ | 50 mM, 1.75fold activation | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | Mycolicibacterium smegmatis | the enzyme is involved in the biosynthesis of L-isoleucine | 2-oxobutanoate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | A0QX60 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-chloro-L-alanine | - |
Mycolicibacterium smegmatis | 3-chloro-2-oxopropionate + NH3 | - |
? | |
| L-allo-threonine | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| L-serine | - |
Mycolicibacterium smegmatis | pyruvate + NH3 | - |
? | |
| L-threonine | - |
Mycolicibacterium smegmatis | 2-oxobutanoate + NH3 | - |
? | |
| L-threonine | the enzyme is involved in the biosynthesis of L-isoleucine | Mycolicibacterium smegmatis | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ilvA | - |
Mycolicibacterium smegmatis |
| MSMEG3183 | - |
Mycolicibacterium smegmatis |
| Threonine deaminase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mycolicibacterium smegmatis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.69 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 9.42 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 27.15 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 32.59 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mycolicibacterium smegmatis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.9 | pH has no significant effect on activity | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Mycolicibacterium smegmatis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the biosynthesis of L-isoleucine | Mycolicibacterium smegmatis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
L-serine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 1.99 | - |
L-allo-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 5.7 | - |
L-threonine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
| 9.02 | - |
beta-chloro-L-alanine | pH 8.0, 30°C | Mycolicibacterium smegmatis | |
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