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Literature summary for 4.3.1.19 extracted from

  • Husain, A.; Jeelani, G.; Sato, D.; Ali, V.; Nozaki, T.
    Characterization of two isotypes of L-threonine dehydratase from Entamoeba histolytica (2010), Mol. Biochem. Parasitol., 170, 100-104.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information insensitive to the allosteric activation by AMP or CMP Entamoeba histolytica

Cloned(Commentary)

Cloned (Comment) Organism
-
Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
2-oxobutanoate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica
glyoxylate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica
L-Cys competitive Entamoeba histolytica
pyruvate 20 mM, about 50% inhibition. Inhibition is not reversed by AMP Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3
-
L-Thr pH 9.0, 37°C Entamoeba histolytica
13.5
-
L-Ser pH 9.0, 37°C Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, calculated Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ser
-
Entamoeba histolytica pyruvate + NH3
-
ir
L-Thr
-
Entamoeba histolytica 2-oxobutanoate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 45000, calculated Entamoeba histolytica

Synonyms

Synonyms Comment Organism
TD1
-
Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
L-Ser pH 9.0, 37°C Entamoeba histolytica
5.2
-
L-Thr pH 9.0, 37°C Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Entamoeba histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1
-
L-Cys substrate L-Ser, pH 9.0, 37°C Entamoeba histolytica
2.2
-
L-Cys substrate L-Thr, pH 9.0, 37°C Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica calculated
-
6.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
L-Ser pH 9.0, 37°C Entamoeba histolytica
0.7
-
L-Thr pH 9.0, 37°C Entamoeba histolytica