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Literature summary for 4.3.1.19 extracted from
- Stability of plant defense proteins in the gut of insect herbivores (2007), Plant Physiol., 143, 1954-1967.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a modified SlTD2 cDNA is cloned into the pET30a+ vector producing a truncated form of SlTD2 lacking the 51 amino acids corresponding to the N-terminal chloroplast-targeting sequence | Solanum lycopersicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
L-threonine | processed TD2 | Solanum lycopersicum |  |
| 3 | - |
L-serine | processed TD2 | Solanum lycopersicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
molecular mass of processed TD2 determined by SDS-PAGE | Solanum lycopersicum |
| 55000 | - |
determined by Western blot analysis, mature TD2 | Solanum lycopersicum |
| 66180 | - |
predicted molecular mass of SlTD1 | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-threonine | Solanum lycopersicum | - |
2-oxobutanoate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | P25306 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Sephadex G-25, a DEAE-cellulose DE52, a HiLoad26/60 Superdex 200 and a HiPrep 16/10 column | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine | - |
Solanum lycopersicum | pyruvate + NH3 | - |
? | |
| L-threonine | - |
Solanum lycopersicum | 2-oxobutanoate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pTD2 | processed form of TD2, the C-terminal regulatory domain is removed | Solanum lycopersicum |
| SlTD1 | isoform, involved in isoleucine biosynthesis in plants | Solanum lycopersicum |
| SlTD2 | isoform, for plant defense the protein is involved in threonine degradation in leaf eating insects | Solanum lycopersicum |
| TD | - |
Solanum lycopersicum |
| TD1 | - |
Solanum lycopersicum |
| TD2 | - |
Solanum lycopersicum |
| Threonine deaminase | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
- |
Solanum lycopersicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
active over a wide range of temperatures, optimal enzyme activity is observed at 58°C | Solanum lycopersicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is highly active in an alkaline pH range, little or no activity is observed at pH values below 6.0 | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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