Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Val | activates | Spinacia oleracea |
General Stability | Organism |
---|---|
L-Ile protects the enzyme against inactivation at low temperatures | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ile | feed-back inhibition; HgCl2 treated enzyme is less sensitive; L-Val partially reverses inhibition | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Spinacia oleracea | |
Li+ | stimulates | Spinacia oleracea | |
NH4+ | stimulates | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
L-Thr | Spinacia oleracea |
7.3 | - |
L-Thr | Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, little loss of activity after 50 days | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Spinacia oleracea | pyruvate + NH3 | - |
? | |
L-threonine | - |
Spinacia oleracea | 2-oxobutanoate + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
10 h, 81% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile | Spinacia oleracea |
27 | - |
10 h, 27% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | tightly bound | Spinacia oleracea | |
pyridoxal 5'-phosphate | cofactor | Spinacia oleracea |