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Literature summary for 4.3.1.19 extracted from

  • Yoshida, H.; Hanada, K.; Ohsawa, H.; Kumagai, H.; Yamada, H.
    Biosynthetic threonine deaminase from Proteus morganii (1982), Agric. Biol. Chem., 46, 1035-1042.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by AMP, ADP and ATP Morganella morganii

Inhibitors

Inhibitors Comment Organism Structure
Ile Hg2+ protects Morganella morganii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
L-Thr
-
Morganella morganii
7.1
-
L-Ser
-
Morganella morganii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
4 * 58000, SDS-PAGE Morganella morganii
228000
-
calculation from sedimentation and diffusion data Morganella morganii
230000
-
gel filtration Morganella morganii

Organism

Organism UniProt Comment Textmining
Morganella morganii
-
biosynthetic threonine deaminase
-
Morganella morganii AKU 0084
-
biosynthetic threonine deaminase
-

Purification (Commentary)

Purification (Comment) Organism
-
Morganella morganii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
318
-
-
Morganella morganii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-Ala
-
Morganella morganii ?
-
?
beta-chloro-L-Ala
-
Morganella morganii AKU 0084 ?
-
?
L-Cys
-
Morganella morganii ?
-
?
L-Cys
-
Morganella morganii AKU 0084 ?
-
?
L-serine
-
Morganella morganii pyruvate + NH3
-
?
L-serine
-
Morganella morganii AKU 0084 pyruvate + NH3
-
?
L-threonine
-
Morganella morganii 2-oxobutanoate + NH3
-
?
L-threonine
-
Morganella morganii AKU 0084 2-oxobutanoate + NH3
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE Morganella morganii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
with L-Thr and L-Ser as substrate Morganella morganii

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11 pH 8.5: about 30% of maximal activity, pH 11.0: about 70% of maximal activity, with L-Thr as substrate Morganella morganii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Morganella morganii
pyridoxal 5'-phosphate cofactor Morganella morganii
pyridoxal 5'-phosphate contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Morganella morganii