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Literature summary for 4.3.1.18 extracted from

  • Deka, G.; Bharath, S.R.; Savithri, H.S.; Murthy, M.R.N.
    Structural and biochemical studies on the role of active site Thr166 and Asp236 in the catalytic function of D-Serine deaminase from Salmonella typhimurium (2018), Biochem. Biophys. Res. Commun., 504, 40-45 .
    View publication on PubMed
Search for more literature for 4.3.1.18

Cloned(Commentary)

Cloned (Comment) Organism
cloned in pET21b vector, expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method at 25°C, 2.7 A resolution crystal structure of mutant enzyme T166A with bound pyridoxal 5'-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
D236L compared to wild-type enzyme and to mutant enzyme T166A the D236L mutant has a lower affinity for pyridoxal 5'-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
T166A in apo-wild-type enzyme and in holo-mutant enzyme T166A structures, which are in the open conformation, Thr166 is about 8-9 A away from a modelled pyridoxal 5'-phosphate D-Ser-external aldimine complex. This distance is too large to function as a proton abstracting residue. Thr166 is important for the proton abstraction step of catalysis Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZL08
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE, mutant enzymes T166A and D236L Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
D-Serine deaminase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Salmonella enterica subsp. enterica serovar Typhimurium