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Literature summary for 4.3.1.18 extracted from
- Reaction mechanism of Zn2+-dependent D-serine dehydratase: role of a conserved tyrosine residue interacting with pyridine ring nitrogen of pyridoxal 5-phosphate (2014), J. Biochem., 156, 173-180.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y203A | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 14% (wild-type 100%) | Saccharomyces cerevisiae |
| Y203D | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 0.57% (wild-type 100%) | Saccharomyces cerevisiae |
| Y203E | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 0.47% (wild-type 100%) | Saccharomyces cerevisiae |
| Y203F | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 25% (wild-type 100%) | Saccharomyces cerevisiae |
| Y203R | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 2.9% (wild-type 100%) | Saccharomyces cerevisiae |
| Y203S | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 14% (wild-type 100%) | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae |  |
| 0.7 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
| 1.8 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
| 3.3 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
| 5.1 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
| 5.6 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
| 8.5 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P53095 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-serine dehydratase | - |
Saccharomyces cerevisiae |
| Dsd1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
| 0.097 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae | |
| 0.5 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
| 2.3 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
| 2.3 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
| 4.3 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
| 17 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae | |
| 0.015 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
| 0.1 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
| 0.68 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
| 1.2 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
| 6.1 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
| 90 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae | |
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