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Literature summary for 4.3.1.18 extracted from
- Role of zinc ion for catalytic activity in D-serine dehydratase from Saccharomyces cerevisiae (2012), FEBS J., 279, 612-624.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C400A | dehydratase activity is completely abolished. Km (beta-chloro-D-alanine) decreased in native and Zn2+-depleted form compared to wild-type. kcat (beta-chloro-D-alanine) increased in native and Zn2+-depleted form compared to wild-type | Saccharomyces cerevisiae |
| H398A | dehydratase activity is completely abolished, kcat (D-serine) highly decreased. Km (beta-chloro-D-alanine) decreased in native and Zn2+-depleted form compared to wild-type. kcat (beta-chloro-D-alanine) increased in native and Zn2+-depleted form compared to wild-type | Saccharomyces cerevisiae |
| K57A | kcat (D-serine) decreased compared to wild-type, no reaction towards beta-chloro-D-alanine | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | removes Zn2+ | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
D-allo-threonine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.039 | - |
D-threonine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.1 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, Zn2+-depleted mutant C400A | Saccharomyces cerevisiae | |
| 0.15 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, native mutant C400A | Saccharomyces cerevisiae | |
| 0.17 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, native mutant H398A | Saccharomyces cerevisiae | |
| 0.19 | - |
D-serine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.75 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, Zn2+-depleted mutant H398A | Saccharomyces cerevisiae | |
| 0.88 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.89 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, wild-type Zn2+-depleted enzyme | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P53095 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-chloro-D-alanine | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-allo-threonine | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? | |
| D-threonine | - |
Saccharomyces cerevisiae | 2-oxobutanoate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-serine dehydratase | - |
Saccharomyces cerevisiae |
| DsdSC | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
D-serine | pH not specified in the publication, 30°C, mutant H398A | Saccharomyces cerevisiae | |
| 0.098 | - |
D-serine | pH not specified in the publication, 30°C, mutant K57A | Saccharomyces cerevisiae | |
| 0.13 | - |
D-allo-threonine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.19 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.32 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, wild-type Zn2+-depleted enzyme | Saccharomyces cerevisiae | |
| 0.39 | - |
D-threonine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
| 0.39 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, Zn2+-depleted mutant H398A | Saccharomyces cerevisiae | |
| 0.69 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, Zn2+-depleted mutant C400A | Saccharomyces cerevisiae | |
| 0.79 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, native mutant H398A | Saccharomyces cerevisiae | |
| 0.96 | - |
beta-Chloro-D-alanine | pH not specified in the publication, 30°C, native mutant C400A | Saccharomyces cerevisiae | |
| 17 | - |
D-serine | pH not specified in the publication, 30°C, wild-type | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
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