Literature summary for 4.3.1.18 extracted from

Bharath, S.R.; Bisht, S.; Savithri, H.S.; Murthy, M.R.
Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium - implications on substrate specificity and catalysis (2011), FEBS J., 278, 2879-2891.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
both wild-type and selenomethionine labelled holoenzyme, to 2.4 and 1.9 A resolution, respectively. Significant electron density is not observed for the cofactor pyridoxal 5'-phosphate. Wild-type enzyme is in an open conformation while selenomethionine enzyme, crystallized in the presence of isoserine, is in a closed conformation suggesting that the enzyme is likely to undergo conformational changes upon binding of substrate. Thr166 may be involved in abstraction of proton from the Calpha atom of the substrate	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

both wild-type and selenomethionine labelled holoenzyme, to 2.4 and 1.9 A resolution, respectively. Significant electron density is not observed for the cofactor pyridoxal 5'-phosphate. Wild-type enzyme is in an open conformation while selenomethionine enzyme, crystallized in the presence of isoserine, is in a closed conformation suggesting that the enzyme is likely to undergo conformational changes upon binding of substrate. Thr166 may be involved in abstraction of proton from the Calpha atom of the substrate

Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.42	-	D-Ser	pH 7.5, 37°C, buffer K+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
0.45	-	D-Thr	pH 7.5, 37°C, buffer Na+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
0.53	-	D-Thr	pH 7.5, 37°C, buffer K+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
0.63	-	D-allo-Thr	pH 7.5, 37°C, buffer Na+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
0.87	-	D-Ser	pH 7.5, 37°C, buffer Na+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
0.88	-	D-allo-Thr	pH 7.5, 37°C, buffer K+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
10.2	-	L-Ser	pH 7.5, 37°C, buffer K+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium
11.8	-	L-Ser	pH 7.5, 37°C, buffer Na+-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.09	-	wild-type, pH 7.5, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.12	-	selenomethionine labelled enzyme, pH 7.5, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-allo-Thr	-	Salmonella enterica subsp. enterica serovar Typhimurium	2-oxobutanoate + NH3	-	?
D-Ser	-	Salmonella enterica subsp. enterica serovar Typhimurium	pyruvate + NH3	-	?
D-Thr	-	Salmonella enterica subsp. enterica serovar Typhimurium	2-oxobutanoate + NH3	-	?
L-Ser	-	Salmonella enterica subsp. enterica serovar Typhimurium	pyruvate + NH3	-	ir

Synonyms

Synonyms	Comment	Organism
DSD	-	Salmonella enterica subsp. enterica serovar Typhimurium