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Literature summary for 4.3.1.18 extracted from

  • Kikuchi, S.; Ishimoto, M.
    A D-serine dehydratase acting also on L-serine from Klebsiella pneumoniae (1978), J. Biochem., 84, 1133-1138.
    View publication on PubMed
Search for more literature for 4.3.1.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 D-Ser
-
 Klebsiella pneumoniae
3.6
-
 D-Thr
-
 Klebsiella pneumoniae
20
-
 L-Ser
-
 Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Klebsiella pneumoniae
46000
-
 gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
 L-Ser Klebsiella pneumoniae
12
-
 D-Ser Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine
-
 Klebsiella pneumoniae pyruvate + NH3
-
 ?
D-threonine
-
 Klebsiella pneumoniae 2-oxobutanoate + NH3
-
 ?
L-serine
-
 Klebsiella pneumoniae pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 30% of maximal activity, pH 8.5: about 60% of maximal activity Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.0025 mM Klebsiella pneumoniae
pyridoxal 5'-phosphate enzyme linked cofactor Klebsiella pneumoniae