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Literature summary for 4.3.1.17 extracted from

  • Xu, X.L.; Grant, G.A.
    Mutagenic and chemical analyses provide new insight into enzyme activation and mechanism of the type 2 iron-sulfur L-serine dehydratase from Legionella pneumophila (2016), Arch. Biochem. Biophys., 596, 108-117 .
    View publication on PubMed
Search for more literature for 4.3.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 DE3 Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Legionella pneumophila

Protein Variants

Protein Variants Comment Organism
C458A kcat/Km for L-serine is 2.2fold lower than the wild-type value Legionella pneumophila
DELTA(C458) mutant enzyme has no detectable activity Legionella pneumophila
DELTA(P456,E457,C458) mutant enzyme has no detectable activity Legionella pneumophila
E445A mutant enzyme has no detectable activity Legionella pneumophila
E457A kcat/Km for L-serine is 1.5fold lower than the wild-type value Legionella pneumophila
H124A/N126A kcat/Km for L-serine is 48.5fold lower than the wild-type value Legionella pneumophila
H19A kcat/Km for L-serine is 127fold lower than the wild-type value Legionella pneumophila
H61A mutant enzyme has no detectable activity Legionella pneumophila
H61N mutant enzyme has no detectable activity Legionella pneumophila
H61S mutant enzyme has no detectable activity Legionella pneumophila
K444A mutant enzyme has no detectable activity Legionella pneumophila
S16A mutant enzyme has no detectable activity Legionella pneumophila
S17A kcat/Km for L-serine is 4.8fold lower than the wild-type value Legionella pneumophila
S18A mutant enzyme has no detectable activity Legionella pneumophila
S53A kcat/Km for L-serine is 2.9fold lower than the wild-type value Legionella pneumophila
T20A kcat/Km for L-serine is 4.2fold lower than the wild-type value Legionella pneumophila
T290A kcat/Km for L-serine is 127fold lower than the wild-type value Legionella pneumophila
T446A kcat/Km for L-serine is 91.7fold lower than the wild-type value Legionella pneumophila
T57A kcat/Km for L-serine is 31.7fold lower than the wild-type value Legionella pneumophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T57A Legionella pneumophila
2
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S17A Legionella pneumophila
2
-
 L-serine pH 7.0, temperature not specified in the publication, native enzyme Legionella pneumophila
2.3
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme E457A Legionella pneumophila
3 3.2 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A Legionella pneumophila
3.1
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T290A Legionella pneumophila
3.5
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S53A Legionella pneumophila
4.7
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T20A Legionella pneumophila
6.9
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme C458A Legionella pneumophila
24.4
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H19A Legionella pneumophila
189
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T446A Legionella pneumophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Legionella pneumophila
-
 pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila Q5ZXE1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Legionella pneumophila pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-serine dehydratase
-
 Legionella pneumophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.07
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T290A Legionella pneumophila
8.8
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T57A Legionella pneumophila
31
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H19A Legionella pneumophila
67
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S17A Legionella pneumophila
112
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A Legionella pneumophila
181
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T20A Legionella pneumophila
201
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S53A Legionella pneumophila
249
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme E457A Legionella pneumophila
330
-
 L-serine pH 7.0, temperature not specified in the publication, native enzyme Legionella pneumophila
356
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T446A Legionella pneumophila
512
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme C458A Legionella pneumophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H19A Legionella pneumophila
1.3
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T290A Legionella pneumophila
1.8
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T446A Legionella pneumophila
3.4
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme H124A/N126A Legionella pneumophila
5.2
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T57A Legionella pneumophila
34
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S17A Legionella pneumophila
39
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme T20A Legionella pneumophila
57
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S53A Legionella pneumophila
74
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme C458A Legionella pneumophila
108
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme E457A Legionella pneumophila
165
-
 L-serine pH 7.0, temperature not specified in the publication, mutant enzyme S16A Legionella pneumophila