Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Rhizomucor miehei |
Crystallization (Comment) | Organism |
---|---|
to 1.76 A resolution. The active site is located between the small domain and large domain, and a pyridoxal 5'-phosphate is attached to Lys52 by a Schiff-base linkage | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | A0A0X1KHE6 | - |
- |
Rhizomucor miehei CAU432 | A0A0X1KHE6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.35 | - |
pH 7.5, 40°C | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Rhizomucor miehei | pyruvate + NH3 | - |
? | |
L-serine | - |
Rhizomucor miehei CAU432 | pyruvate + NH3 | - |
? | |
L-threonine | - |
Rhizomucor miehei | 2-oxobutanoate + NH3 | - |
? | |
L-threonine | - |
Rhizomucor miehei CAU432 | 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | * 41000, SDS-PAGE | Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhizomucor miehei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhizomucor miehei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Rhizomucor miehei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | cofactor is located in the crevice between the two domains, attached to residue Lys52 by a Schiff-base linkage | Rhizomucor miehei |