Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00258 | - |
L-serine | pH 7, temperature not specified in the publication, Vmax: 11.7/min/mg | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35680 | - |
His-tagged fusion protein, calculated from cDNA | Mycolicibacterium smegmatis |
57000 | - |
gel filtration, dimer | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycolicibacterium smegmatis | cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QY48 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Mycolicibacterium smegmatis | pyruvate + NH3 | - |
? | |
additional information | cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 | Mycolicibacterium smegmatis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
MSMEG_3532 | - |
Mycolicibacterium smegmatis |
SdhA | - |
Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
physiological function | cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 | Mycolicibacterium smegmatis |