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Literature summary for 4.3.1.17 extracted from
- Allosteric activation and contrasting properties of L-serine dehydratase types 1 and 2 (2012), Biochemistry, 51, 5320-5328.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Bacillus subtilis |
| expressed in Escherichia coli as a His-tagged fusion protein | Legionella pneumophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibition | Bacillus subtilis |  |
| Co2+ | inhibition | Legionella pneumophila | |
| Ni2+ | inhibition | Bacillus subtilis | |
| Ni2+ | inhibition | Legionella pneumophila | |
| oxygen | bsLSD is highly sensitive to oxygen | Bacillus subtilis | |
| oxygen | lpLSD is weakly sensitive to oxygen | Legionella pneumophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
L-serine | pH 7, temperature not specified in the publication | Legionella pneumophila | |
| 10.6 | - |
L-serine | pH 7, temperature not specified in the publication | Bacillus subtilis | |
| 13.6 | - |
L-serine | pH 7, temperature not specified in the publication, 100 mM KCl | Bacillus subtilis | |
| 25.5 | - |
L-serine | pH 7, temperature not specified in the publication, 300 mM KCl | Bacillus subtilis | |
| 29.2 | - |
L-serine | pH 7, temperature not specified in the publication, 500 mM KCl | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | addition results in weak activation | Legionella pneumophila | |
| K+ | required, addition results in strong activation | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Legionella pneumophila | Q5ZXE1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using cobalt-based affinity columns | Bacillus subtilis |
| using cobalt-based affinity columns | Legionella pneumophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine | - |
Bacillus subtilis | pyruvate + NH3 | - |
? | |
| L-serine | - |
Legionella pneumophila | pyruvate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bsLSD type 1 | - |
Bacillus subtilis |
| L-serine dehydratase | - |
Bacillus subtilis |
| L-serine dehydratase | - |
Legionella pneumophila |
| lpLSD type 2 | - |
Legionella pneumophila |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
L-serine | pH 7, temperature not specified in the publication | Bacillus subtilis | |
| 184 | - |
L-serine | pH 7, temperature not specified in the publication, 100 mM KCl | Bacillus subtilis | |
| 286 | - |
L-serine | pH 7, temperature not specified in the publication | Legionella pneumophila | |
| 465 | - |
L-serine | pH 7, temperature not specified in the publication, 300 mM KCl | Bacillus subtilis | |
| 585 | - |
L-serine | pH 7, temperature not specified in the publication, 500 mM KCl | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus subtilis |
| 7 | - |
assay at | Legionella pneumophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Bacillus subtilis |
| 6 | 10 | - |
Legionella pneumophila |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
L-serine | pH 7, temperature not specified in the publication | Bacillus subtilis | |
| 14 | - |
L-serine | pH 7, temperature not specified in the publication, 100 mM KCl | Bacillus subtilis | |
| 18 | - |
L-serine | pH 7, temperature not specified in the publication, 300 mM KCl | Bacillus subtilis | |
| 20 | - |
L-serine | pH 7, temperature not specified in the publication, 500 mM KCl | Bacillus subtilis | |
| 57 | - |
L-serine | pH 7, temperature not specified in the publication | Legionella pneumophila | |
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