Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutyrate | 20 mM: 49.3% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica | |
ATP | - |
Entamoeba histolytica | |
glyoxylate | 20 mM: 54.1% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica | |
L-cysteine | competitive | Entamoeba histolytica | |
pyruvate | 20 mM: 45.3% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
L-threonine | - |
Entamoeba histolytica | |
13.5 | - |
L-serine | - |
Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, calculated | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | B1N2N4 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Entamoeba histolytica | pyruvate + NH3 | - |
? | |
L-threonine | - |
Entamoeba histolytica | 2-oxobutyrate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, calculated | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
L-serine dehydratase | - |
Entamoeba histolytica |
L-threonine dehydratase | - |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-serine | - |
Entamoeba histolytica | |
5.2 | - |
L-threonine | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
L-cysteine | substrate L-serine | Entamoeba histolytica | |
2.2 | - |
L-cysteine | substrate L-threonine | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | calculated | - |
6.68 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
10 | - |
- |
Entamoeba histolytica | ATP |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-serine | - |
Entamoeba histolytica | |
0.7 | - |
L-threonine | - |
Entamoeba histolytica |