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Literature summary for 4.3.1.15 extracted from

  • Rupesh, K.R.; PremKumar, P.L.; Kumar, V.V.S.; Jayachandran, S.S.
    Production of diamino propionic acid ammonia lyase by a new strain of Salmonella typhimurium PU011 (2002), BMC Microbiol., 2, 5.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.685
-
 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide pH 8.0, 28°C Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 PU011, produces enzyme when grown on minimal medium containing 2,3-diaminoproprionic acid
-

Purification (Commentary)

Purification (Comment) Organism
partial Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-[5-amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
L-2,3-Diaminopropanoate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium Pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 sharp Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
6 11 drastic reduction in activity above pH 11, below pH 6 Salmonella enterica subsp. enterica serovar Typhimurium