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Literature summary for 4.3.1.14 extracted from

  • Jeng, I.M.; Barker, H.A.
    Purification and properties of L-3-aminobutyryl coenzyme A deaminase from a lysine-fermenting Clostridium (1974), J. Biol. Chem., 249, 6578-6584.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6,6'-Dithiodinicotinic acid
-
Clostridium sp.
CaCl2 2 mM, 28% inhibition Clostridium sp.
iodoacetamide crotonyl-CoA protects Clostridium sp.
KCl 400 mM, 32% inhibition Clostridium sp.
MgCl2 2 mM, 22% inhibition Clostridium sp.
MgSO4 2 mM, 22% inhibition Clostridium sp.
NaCl 400 mM, 34% inhibition Clostridium sp.
NEM
-
Clostridium sp.
PCMB
-
Clostridium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
L-3-aminobutyryl-CoA
-
Clostridium sp.
0.045
-
crotonyl-CoA
-
Clostridium sp.
0.07
-
NH3 with crotonyl-CoA as cosubstrate Clostridium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration Clostridium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium sp. enzyme is involved in the pathway of Lys degradation ?
-
?
additional information Clostridium sp. SB4 enzyme is involved in the pathway of Lys degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
SB4
-
Clostridium sp. SB4
-
SB4
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.37
-
-
Clostridium sp.

Storage Stability

Storage Stability Organism
-20°C, 3 mg/ml enzyme concentration, 4% loss of activity in 3 months Clostridium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is involved in the pathway of Lys degradation Clostridium sp. ?
-
?
additional information enzyme is involved in the pathway of Lys degradation Clostridium sp. SB4 ?
-
?
L-3-Aminobutanoyl-CoA r Clostridium sp. Crotonyl-CoA + NH3
-
?
L-3-Aminobutanoyl-CoA only active with the L-isomer Clostridium sp. Crotonyl-CoA + NH3
-
?
L-3-Aminobutanoyl-CoA r Clostridium sp. SB4 Crotonyl-CoA + NH3
-
?
L-3-Aminobutanoyl-CoA only active with the L-isomer Clostridium sp. SB4 Crotonyl-CoA + NH3
-
?
Crotonyl pantetheine + NH3 aminated 100% as fast as crotonyl-CoA Clostridium sp. Aminobutanoylpantetheine
-
?
Crotonyl pantetheine + NH3 aminated 100% as fast as crotonyl-CoA Clostridium sp. SB4 Aminobutanoylpantetheine
-
?
Crotonyl-CoA + hydroxylamine
-
Clostridium sp. CoA + 3-methyl-5-isoxazolidinone
-
?
Crotonyl-CoA + hydroxylamine
-
Clostridium sp. SB4 CoA + 3-methyl-5-isoxazolidinone
-
?
L-3-aminobutyryl-CoA
-
Clostridium sp. crotonoyl-CoA + NH3
-
?
crotonoyl-CoA + NH3 NH3 in form of NH4Cl Clostridium sp. L-3-aminobutyryl-CoA
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 6.8, half-life: 7.5 h, 0.05 mg of protein per ml Clostridium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
amination Clostridium sp.
9.7
-
deamination Clostridium sp.