Literature summary for 4.3.1.13 extracted from

  • Cooper, A.J.L.; Meister, A.
    Enzymatic conversion of O-carbamyl-L-serine to pyruvate and ammonia (1973), Biochem. Biophys. Res. Commun., 55, 780-787.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
beta-chloro-L-alanine irreversible Rattus norvegicus
L-Albizziin 20 mM, 20% inhibition Rattus norvegicus
L-glutamine 20 mM, 60% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
O-Carbamoyl-L-serine
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-Carbamoyl-L-serine Rattus norvegicus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.49
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, no loss of activity after 2 weeks in 20% glycerol containing 0.1 mM pyridoxal 5-phosphate, 1 mM 2-mercaptoethanol and 10 mM potassium phosphate bufer, pH 7.2 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-carbamoyl-L-serine
-
Rattus norvegicus pyruvate + 2 NH3 + CO2
-
?
S-carbamoyl-L-cysteine
-
Rattus norvegicus pyruvate + ? 10% pyruvate formation of that towards O-carbamoyl-L-serine ?
O-Carbamoyl-L-serine
-
Rattus norvegicus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
enzyme assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.8 in 0.1 M sodium borate buffer Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate omission leads to 60% decrease of activity Rattus norvegicus