Application | Comment | Organism |
---|---|---|
biotechnology | a complete biocatalytic process to synthesize high concentrations of L-aspartate catalyzed by aspartase from Bacillus sp. YM55-1 (AspB) is established using an immobilized enzyme in three different supports. MANA-agarose derivative could be selected as the most suitable biocatalyst for the synthesis of Asp due to the simplicity of the method and performance | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
AspB is expressed in Escherichia coli | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
213 | - |
fumarate | co-substrate: NH3, pH 7.5, 37°, free-enzyme | Bacillus sp. (in: Bacteria) | |
213 | - |
fumarate | co-substrate: NH3, pH 7.5, 37°, LentiKats-immobilized enzyme | Bacillus sp. (in: Bacteria) | |
244 | - |
fumarate | co-substrate: NH3, pH 7.5, 37°, EupergitC-immobilized enzyme | Bacillus sp. (in: Bacteria) | |
244 | - |
fumarate | co-substrate: NH3, pH 7.5, 37°, MANA-agarose-immobilized enzyme | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q9LCC6 | - |
- |
Bacillus sp. (in: Bacteria) YM55-1 | Q9LCC6 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified by heat precipitation and saturation with ammonium sulfate | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
AspB is immobilized by covalent attachment on Eupergit (epoxy support) and MANAagarose (amino support), and entrapment in LentiKats (polyvinyl alcohol) with retained activities of 24, 85 and 63%, respectively | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + NH3 | - |
Bacillus sp. (in: Bacteria) | L-aspartate | - |
? | |
fumarate + NH3 | - |
Bacillus sp. (in: Bacteria) YM55-1 | L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
aspB | - |
Bacillus sp. (in: Bacteria) |
L-aspartate ammonia-lyase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Bacillus sp. (in: Bacteria) |