Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.1 extracted from

  • Viola, R.E.; Yerman, L.; Fowler, J.M.; Arvidson, C.G.; Brubaker, R.R.
    A missense mutation causes aspartase deficiency in Yersinia pestis (2008), Microbiology, 154, 1271-1280.
    View publication on PubMed
Search for more literature for 4.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
functional and inactive aspA are cloned and expressed in AspA-deficient Escherichia coli Yersinia pseudotuberculosis
functional and inactive aspA of Yersinia pestis KIM is cloned and expressed in AspA-deficient Escherichia coli Yersinia pestis

Protein Variants

Protein Variants Comment Organism
L363V single mutant Yersinia pestis
V363L mutation in primary structure causing dramatic differences in catalytic activity do not promote significant changes in secondary structure Yersinia pseudotuberculosis
Y146D single mutant Yersinia pestis
Y146D/L363V double mutant Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 L-aspartate double mutant L363V/Y146D Yersinia pestis
1.3
-
 L-aspartate mutant Y146D1 Yersinia pestis
1.3
-
 L-aspartate recmbinant enzyme Yersinia pestis
1.5
-
 L-aspartate mutant L363V Yersinia pestis
2.1
-
 L-aspartate
-
 Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ included in assay medium Yersinia pseudotuberculosis
Mg2+ included in assay medium Yersinia pestis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52470
-
 mass of monomer, determined by an ESI-qTOF mass spectrometer Yersinia pseudotuberculosis
52540
-
 mass of monomer, determined by an ESI-qTOF mass spectrometer Yersinia pestis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Yersinia pseudotuberculosis
-
 fumarate + NH3 fumarate is a component of the tricarboxylic acid (TCA) cycle ?
L-aspartate Yersinia pestis
-
 fumarate + NH3 fumarate is a component of the tricarboxylic acid (TCA) cycle ?

Organism

Organism UniProt Comment Textmining
Yersinia pestis
-
-
-
Yersinia pseudotuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns Yersinia pestis
transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in purification buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns Yersinia pseudotuberculosis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture strain KIM, possesses a base transversion (GC-TA) at position 146 of AspA, causing replacement of aspartate by tyrosine Yersinia pestis
-
cell culture strain PB1 of serogroup 1b Yersinia pseudotuberculosis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity below 0.001, pCD- (lacking low-calcium response plasmid into pseudogenes) Yersinia pestis
additional information
-
 activity below 0.001, pCD- (lacking low-calcium response plasmid into pseudogenes), 4 mM Ca2+ Yersinia pestis
additional information
-
 activity below 0.001, pCD+ (containing low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 Yersinia pestis
additional information
-
 activity below 0.001, pCD+ (containing low-calcium response plasmid into pseudogenes), 4 mM Ca2+ Yersinia pestis
0.044
-
 pYV- (lacking low-calcium response plasmid into pseudogenes), 4 mM Ca2+, 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 Yersinia pseudotuberculosis
0.05
-
 pYV- (lacking low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 Yersinia pseudotuberculosis
0.06
-
 pYV+ (containing low-calcium response plasmid into pseudogenes), 4 mM Ca2+, 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 Yersinia pseudotuberculosis
0.075
-
 pYV+ (containing low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 Yersinia pseudotuberculosis
0.2
-
 mutant L363V, 50 mM HEPES, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate Yersinia pestis
0.2
-
 mutant Y146D, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate Yersinia pestis
0.2
-
 recombinant enzyme, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate Yersinia pestis
72
-
 double mutant L363V/Y146D, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate Yersinia pestis
80
-
 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
 Yersinia pseudotuberculosis fumarate + NH3 fumarate is a component of the tricarboxylic acid (TCA) cycle ?
L-aspartate
-
 Yersinia pestis fumarate + NH3 fumarate is a component of the tricarboxylic acid (TCA) cycle ?

Subunits

Subunits Comment Organism
homotetramer predicted and determined by dynamic light scattering (DLS), loss of activity in mutants reflects significant changes in subunit association. Yersinia pestis

Synonyms

Synonyms Comment Organism
ASPA
-
 Yersinia pseudotuberculosis
ASPA
-
 Yersinia pestis
aspartase
-
 Yersinia pseudotuberculosis
aspartase
-
 Yersinia pestis
aspartate ammonia-lyase
-
 Yersinia pseudotuberculosis
aspartate ammonia-lyase
-
 Yersinia pestis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 L-aspartate recombinant enzyme Yersinia pestis
0.12
-
 L-aspartate mutant L363V Yersinia pestis
0.13
-
 L-aspartate mutant Y146D Yersinia pestis
86
-
 L-aspartate double mutant L363V/Y146D Yersinia pestis
87
-
 L-aspartate
-
 Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 AspA assay in cell preparations Yersinia pseudotuberculosis
7
-
 AspA assay in cell preparations Yersinia pestis
8
-
 AspA assay in purified preparations Yersinia pseudotuberculosis
8
-
 AspA assay in purified preparations Yersinia pestis