Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2 mM, 98% inhibition | Cytophaga sp. | |
Hg2+ | 2 mM, 98% inhibition | Cytophaga sp. | |
iodoacetate | 1 mM, 94% inhibition | Cytophaga sp. | |
K+ | 2 mM, 98% inhibition | Cytophaga sp. | |
N-ethylmaleimide | 1 mM, 100% inhibition | Cytophaga sp. | |
Na+ | 2 mM, 98% inhibition | Cytophaga sp. | |
Ni2+ | 2 mM, 98% inhibition | Cytophaga sp. | |
p-chloromercuribenzoate | 0.1 mM, 100% inhibition | Cytophaga sp. | |
Semicarbazide hydrochloride | 1 mM, 30% inhibition | Cytophaga sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.797 | - |
fumarate | 0.1 mM, 100% inhibition | Cytophaga sp. | |
1.09 | - |
L-aspartate | pH 7.0, 55°C | Cytophaga sp. | |
25.2 | - |
NH3 | 0.1 mM, 100% inhibition | Cytophaga sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation at neutral pH | Cytophaga sp. | |
Mg2+ | activation at neutral pH | Cytophaga sp. | |
Mn2+ | activation at neutral pH | Cytophaga sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Cytophaga sp. |
192000 | - |
gel filtration | Cytophaga sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cytophaga sp. | - |
KUC-1 | - |
Cytophaga sp. KUC-1 | - |
KUC-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Cytophaga sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
301 | - |
pH 8.5, 30°C | Cytophaga sp. |
Storage Stability | Organism |
---|---|
-20°C, pH 7.0, 2% 2-mercaptoethanol, several months, no loss of activity | Cytophaga sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + hydroxylamine | at 64% the rate of NH3 | Cytophaga sp. | L-aspartate | - |
? | |
fumarate + hydroxylamine | at 64% the rate of NH3 | Cytophaga sp. KUC-1 | L-aspartate | - |
? | |
fumarate + NH3 | - |
Cytophaga sp. | L-aspartate | - |
? | |
fumarate + NH3 | - |
Cytophaga sp. KUC-1 | L-aspartate | - |
? | |
L-aspartate | - |
Cytophaga sp. | fumarate + NH3 | - |
r | |
L-aspartate | - |
Cytophaga sp. KUC-1 | fumarate + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 51000, SDS-PAGE | Cytophaga sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Cytophaga sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | - |
Cytophaga sp. |
50 | - |
60 min, 80% residual activity | Cytophaga sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
absence of Mg2+ | Cytophaga sp. |
8.5 | - |
presence of Mg2+ | Cytophaga sp. |