Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-Aspartate | - |
Escherichia coli | |
o-phospho-D-serine | - |
Escherichia coli |
Application | Comment | Organism |
---|---|---|
biotechnology | overview on commercial applications | Escherichia coli |
medicine | involvement of enzyme in blood clotting and activation of plasminogen, overview | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C141S/C274A | elimination of sensitivity to inactivation | Escherichia coli |
K140I | Km value 10fold higher than wild type, comparable increase in Ki for competitive inhibitors | Escherichia coli |
additional information | overview | Escherichia coli |
additional information | overview | Hafnia alvei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | - |
Escherichia coli | |
3-nitropropanoate | - |
Escherichia coli | |
D-malate | - |
Escherichia coli | |
DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
additional information | overview on inhibitors | Escherichia coli | |
o-phospho-D-serine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Hafnia alvei | |
Mn2+ | activation | Hafnia alvei | |
additional information | absolute requirement for divalent metal ion at higher pH | Escherichia coli | |
additional information | no activation by Ca2+, minimal activation by Co2+, pH-dependent activation by divalent cations at high pH values | Hafnia alvei | |
Zn2+ | activation | Hafnia alvei | |
Zn2+ | most potent activating metal ion | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Escherichia coli |
52000 | - |
4 * 52000, overview | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Hafnia alvei | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overview | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate = fumarate + NH3 | mechanism | Escherichia coli | |
L-aspartate = fumarate + NH3 | mechanism: overview | Hafnia alvei |
Renatured (Comment) | Organism |
---|---|
denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Escherichia coli |
denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Escherichia coli | fumarate + NH3 | - |
r | |
additional information | no activity with D-aspartic acid or crotonic acid, overview substrate specificity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52000, overview | Pseudomonas fluorescens |
More | overview | Hafnia alvei |
tetramer | 4 * 52000, SDS-PAGE | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
o-phospho-D-serine | - |
Escherichia coli | |
0.66 | - |
D-malate | - |
Escherichia coli | |
0.66 | - |
DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
0.83 | - |
3-nitropropanoate | - |
Escherichia coli | |
1.1 | - |
2,3-diphosphoglycerate | - |
Escherichia coli |