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Literature summary for 4.2.99.B1 extracted from
- Modulation of the 5'-deoxyribose-5-phosphate lyase and DNA synthesis activities of mammalian DNA polymerase beta by apurinic/apyrimidinic endonuclease 1 (2004), J. Biol. Chem., 279, 25268-25275.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| apurinic/apyrimidinic endonuclease 1 | i.e. APE1 or AP endonulease 1, stimulates 5'-dRP excision by a mechanism independent of its apurinic/apyrimidinic endonuclease activity. 10-15% increase of activity in reactions containing both EDTA and APE1. No stimulation in presence of MgCl2. The mutant form R177A of APE1 and wild-type show similar stimulation effects on dRP lyase activity | Homo sapiens | |
| dATP | at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates | Homo sapiens |  |
| dCTP | at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates. Apparent KM for dCTP on a single-nucleotide gapped DNA as substrate: 0.2 microM. It is suggested that the mechanism of stimulation may involve either Pol beta protein conformational changes upon nucleoside triphosphate binding or altered protein-DNA interactions accomanying dCMP incorporation | Homo sapiens | |
| dGTP | at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates | Homo sapiens | |
| dUTP | at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | in reaction buffer HEPES-KOH, pH 7.5, without EDTA, enhanced enzyme activity, when 20 microM dCTP is included. No stimulation of enzyme activity with MgCl2, dCTP, and EDTA | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site | DNA polymerase beta (Pol beta) catalyzes both DNA synthesis at the 3'-hydroxyl terminus and esxcision of 5'-dRP moiety prior to completion of the base excsion repair (BER) by DNA ligase | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-deoxyribose-5-phosphate lyase | - |
Homo sapiens |
| pol beta | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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