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Literature summary for 4.2.99.23 extracted from
- Molecular identification of tuliposide B-converting enzyme: a lactone-forming carboxylesterase from the pollen of tulip (2015), Plant J., 83, 252-262.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta 2 (DE3) cells | Tulipa gesneriana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Tulipa gesneriana |  |
| Cu2+ | - |
Tulipa gesneriana | |
| Fe2+ | - |
Tulipa gesneriana | |
| Hg2+ | - |
Tulipa gesneriana | |
| phenylmethylsulfonyl fluoride | 2 mM inhibits the activity of isoforms 1, 2 and 3 by 89, 99.7 and 90.2%, respectively | Tulipa gesneriana | |
| V3+ | - |
Tulipa gesneriana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
6-tuliposide A | isoform 2, pH and temperature not specified in the publication | Tulipa gesneriana | |
| 1.8 | - |
6-tuliposide A | isoform 1, pH and temperature not specified in the publication | Tulipa gesneriana | |
| 3 | - |
6-tuliposide A | isoform 3, pH and temperature not specified in the publication | Tulipa gesneriana | |
| 7.4 | - |
6-tuliposide B | isoform 3, pH and temperature not specified in the publication | Tulipa gesneriana | |
| 8.7 | - |
6-tuliposide B | isoform 2, pH and temperature not specified in the publication | Tulipa gesneriana | |
| 9.5 | - |
6-tuliposide B | isoform 1, pH and temperature not specified in the publication | Tulipa gesneriana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Tulipa gesneriana | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, isoform 1, 2, and 3, SDS-PAGE | Tulipa gesneriana |
| 92000 | - |
isoform 2, gel filtration | Tulipa gesneriana |
| 93000 | - |
isoform 3, gel filtration | Tulipa gesneriana |
| 99000 | - |
isoform 1, gel filtration | Tulipa gesneriana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-tuliposide B | Tulipa gesneriana | preferred substrate | tulipalin B + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tulipa gesneriana | - |
cultivar Murasakizuisho | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Tulipa gesneriana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | the enzyme is specifically expressed in the pollen grains, but not in the anther cells | Tulipa gesneriana | - |
| stamen | - |
Tulipa gesneriana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-tuliposide A | the activities of isoforms 1, 2 and 3 towards 6-tuliposide A are 0.41, 1.8, and 1.8% of those towards P6-tuliposide B, respectively | Tulipa gesneriana | tulipalin A + D-glucose | - |
? | |
| 6-tuliposide B | preferred substrate | Tulipa gesneriana | tulipalin B + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 47000, isoform 1, 2, and 3, SDS-PAGE | Tulipa gesneriana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PosB-converting enzyme | - |
Tulipa gesneriana |
| TCEB | - |
Tulipa gesneriana |
| TCEB1 | - |
Tulipa gesneriana |
| tuliposide B-converting enzyme | - |
Tulipa gesneriana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Tulipa gesneriana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | the stable temperatures of the enzymes are up to 45°C for isoforms 1 and 2, and 50°C for isoform 3 | Tulipa gesneriana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Tulipa gesneriana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | isoforms 1, 2 and 3 are stable in the pH range of 4.5-10.0 | Tulipa gesneriana |
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Release 2023.1 (January 2023)
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