Literature summary for 4.2.99.18 extracted from

Redrejo-Rodriguez, M.; Ishchenko, A.A.; Saparbaev, M.K.; Salas, M.L.; Salas, J.
African swine fever virus AP endonuclease is a redox-sensitive enzyme that repairs alkylating and oxidative damage to DNA (2009), Virology, 390, 102-109.

Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BH110	African swine fever virus

Inhibitors

Inhibitors	Comment	Organism	Structure
beta-mercaptoethanol	complete inhibition at 10 mM and higher	African swine fever virus
dithiothreitol	at 1 mM and higher dithiothreitol concentrations AP site incision is strongly inhibited with less than 10% of the activity remaining, the inhibition by dithiothreitol is reverted by H2O2 in a dose-dependent manner, the inhibition by dithiothreitol is not reverted by divalent cations, including Zn2+, Co2+, Ca2+, and Ni+	African swine fever virus

Organism

Organism	UniProt	Comment	Textmining
African swine fever virus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and HisTrap chelating column chromatography	African swine fever virus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA	AP endonuclease pE296R has strong preference for mispaired and oxidative base lesions at the 3'-termini of single-strand breaks, the 3'-terminal damaged pyrimidines (uracil, 5,6-dihydrouracil, and 5-hydroxycytosine) are removed with higher efficiency than damaged purines inosine and 7,8-dihydro-8-oxoguanine	African swine fever virus	fragments of DNA	-	?

Synonyms

Synonyms	Comment	Organism
AP endonuclease	-	African swine fever virus
apurinic-apyrimidinic endonuclease	-	African swine fever virus
pE296R	-	African swine fever virus

General Information

General Information	Comment	Organism
physiological function	AP endonuclease pE296R is essential for virus growth in swine macrophages. DNA repair functions of pE296R are AP endonucleolytic, 3'-5' exonuclease, 3-diesterase and nucleotide incision repair activities	African swine fever virus

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Release 2023.1 (January 2023)