Cloned (Comment) | Organism |
---|---|
Human Ape1/Ref-1 cDNA is amplified by RT-PCR using Ape1/Ref-1-specific primers and from human GM00637 fibroblasts. AP endonuclease domain deletion mutant deltaAPD is amplified by RT-PCR using specific primers from full length human Ape1/Ref-1 cDNA. Redox domain deletion mutant deltaRD is amplified by RT-PCR using specific primers from full length human Ape1/Ref-1 cDNA. | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme cleaves the AP sites in DNA and allows them to be repaired by other enzymes involved in base excision repair | ? | - |
? | |
additional information | Homo sapiens | enzyme stimulates the DNA binding activity of the AP-1 family of transcription factors via a redox-dependent mechanism | ? | - |
? | |
additional information | Homo sapiens | the DNA-binding ability of NF-kappaB in the Ape1/Ref-1 expressing cells is significantly increased | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
GM00637 cell | fibroblasts | Homo sapiens | - |
pancreatic cancer cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Ape1/Ref-1 increases the expression of glial cell-derived neurotropic factor receptor alpha1 (GFRalpha1), a key receptor for glial cell-derived neurotropic factor (GDNF). Expression of Ape1/Ref- 1 leads to an increase in the GDNF responsiveness in human fibroblast. Ape1/Ref-1 induced GFRalpha1 transcription through enhances binding of NF-kappaB complexes to the GFRalpha1 promoter. | Homo sapiens |
additional information | - |
GFRalpha1 levels correlates proportionally with Ape1/Ref-1 in cancer cells | Homo sapiens |
additional information | - |
in neuronal cells, the Ape1/Ref-1-mediated increase in GDNF responsiveness not only stimulated neurite outgrowth but also protected the cells from beta-amyloid peptide and oxidative stress | Homo sapiens |
additional information | - |
the knockdown of endogenous Ape1/Ref-1 in pancreatic cancer cells markedly suppresses GFRalpha1 expression and invasion in response to GNDF, while overexpression of GFRalpha1 restores invasion | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme cleaves the AP sites in DNA and allows them to be repaired by other enzymes involved in base excision repair | Homo sapiens | ? | - |
? | |
additional information | enzyme stimulates the DNA binding activity of the AP-1 family of transcription factors via a redox-dependent mechanism | Homo sapiens | ? | - |
? | |
additional information | the DNA-binding ability of NF-kappaB in the Ape1/Ref-1 expressing cells is significantly increased | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1/Ref-1 | - |
Homo sapiens |
apurinic/apyrimidinic endonuclease 1 | - |
Homo sapiens |
redox factor-1:Ref-1 | - |
Homo sapiens |