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Literature summary for 4.2.99.18 extracted from

  • Izumi, T.; Schein, C.H.; Oezguen, N.; Feng, Y.; Braun, W.
    Effects of backbone contacts 3' to the abasic site on the cleavage and the product binding by human apurinic/apyrimidinic endonuclease (APE1) (2004), Biochemistry, 43, 684-689.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
N226A increased cleavage rate at apurinic/apyrimidinic sites, ability to bind to damaged DNA decreases Homo sapiens
N226A/N229A ability of the mutant to bind damaged DNA is decreased, Vmax is almost identical to that of the wild-type enzyme Homo sapiens
N229A increased cleavage rate at apurinic/apyrimidinic sites, ability to bind to damaged DNA decreases Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000034
-
43-mer oligonucleotide containing apurinic/apyrimidinic sites 37°C, wild-type enzyme Homo sapiens
0.0000136
-
43-mer oligonucleotide containing apurinic/apyrimidinic sites 37°C, mutant enzyme N226A Homo sapiens
0.0000184
-
43-mer oligonucleotide containing apurinic/apyrimidinic sites 37°C, mutant enzyme N229A Homo sapiens
0.0000278
-
43-mer oligonucleotide containing apurinic/apyrimidinic sites 37°C, mutant enzyme N226A/N229A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing apurinic/apyrimidinic sites Homo sapiens hydrogen bonds to phosphate groups 3' to the cleavage site is essential for the binding of the enzyme to the product DNA, which may be necessary for efficient functioning of the base excision rapair pathway fragments of DNA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27695
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
43-mer oligonucleotide containing apurinic/apyrimidinic sites
-
Homo sapiens fragments of DNA
-
?
DNA containing apurinic/apyrimidinic sites hydrogen bonds to phosphate groups 3' to the cleavage site is essential for the binding of the enzyme to the product DNA, which may be necessary for efficient functioning of the base excision rapair pathway Homo sapiens fragments of DNA
-
?

Synonyms

Synonyms Comment Organism
APE1
-
Homo sapiens
apurinic/apyrimidinic endonuclease
-
Homo sapiens
nuclease SmnA
-
Homo sapiens