Protein Variants | Comment | Organism |
---|---|---|
N226A | increased cleavage rate at apurinic/apyrimidinic sites, ability to bind to damaged DNA decreases | Homo sapiens |
N226A/N229A | ability of the mutant to bind damaged DNA is decreased, Vmax is almost identical to that of the wild-type enzyme | Homo sapiens |
N229A | increased cleavage rate at apurinic/apyrimidinic sites, ability to bind to damaged DNA decreases | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000034 | - |
43-mer oligonucleotide containing apurinic/apyrimidinic sites | 37°C, wild-type enzyme | Homo sapiens | |
0.0000136 | - |
43-mer oligonucleotide containing apurinic/apyrimidinic sites | 37°C, mutant enzyme N226A | Homo sapiens | |
0.0000184 | - |
43-mer oligonucleotide containing apurinic/apyrimidinic sites | 37°C, mutant enzyme N229A | Homo sapiens | |
0.0000278 | - |
43-mer oligonucleotide containing apurinic/apyrimidinic sites | 37°C, mutant enzyme N226A/N229A | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing apurinic/apyrimidinic sites | Homo sapiens | hydrogen bonds to phosphate groups 3' to the cleavage site is essential for the binding of the enzyme to the product DNA, which may be necessary for efficient functioning of the base excision rapair pathway | fragments of DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27695 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
43-mer oligonucleotide containing apurinic/apyrimidinic sites | - |
Homo sapiens | fragments of DNA | - |
? | |
DNA containing apurinic/apyrimidinic sites | hydrogen bonds to phosphate groups 3' to the cleavage site is essential for the binding of the enzyme to the product DNA, which may be necessary for efficient functioning of the base excision rapair pathway | Homo sapiens | fragments of DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |
apurinic/apyrimidinic endonuclease | - |
Homo sapiens |
nuclease SmnA | - |
Homo sapiens |