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Literature summary for 4.2.99.18 extracted from

  • Spiering, A.L.; Deutsch, W.A.
    Drosophila apurinic/apyrimidinic DNA endonucleases. Characterization of mechanism of action and demonstration of a novel type of enzyme activation (1986), J. Biol. Chem., 261, 3222-3228.
    View publication on PubMed
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 1 * 63000, AP endonuclease II, SDS-PAGE Drosophila melanogaster
66000
-
 1 * 66000, AP endonuclease I, SDS-PAGE Drosophila melanogaster
67000
-
 AP endonuclease II, glycerol density gradient sedimentation Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Drosophila melanogaster
-
 fragments of DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
AP endonuclease I and II, 400fold Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
 Drosophila melanogaster fragments of DNA
-
 ?
DNA AP endonuclease I forms deoxyribose 3'-phosphate and 5'-OH termini upon cleaving depurinated DNA Drosophila melanogaster fragments of DNA
-
 ?
DNA cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site Drosophila melanogaster fragments of DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, AP endonuclease I, SDS-PAGE Drosophila melanogaster
monomer 1 * 63000, AP endonuclease II, SDS-PAGE Drosophila melanogaster