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Literature summary for 4.2.99.18 extracted from
- Characterization of Escherichia coli x-ray endonuclease, endonulease II (1983), Biochemistry, 22, 4071-4081.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by N-ethylmaleimide | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulates | Escherichia coli |  |
| KCl | optimal concentration: 0.05-0.1 M, activity against apurinic and apyrimidinic sites, 50% inhibition at 0.02 and 0.12 M | Escherichia coli | |
| KCl | optimal concentration: 0.10 M, activity against OsO4-sites, 50% inhibition at 0.05 and 0.15 M | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
glycerol density gradient sedimentation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Escherichia coli | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
X-ray endonuclease III | - |
| Escherichia coli | - |
enzyme also has DNA N-glycosylase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Escherichia coli | fragments of DNA | - |
? | |
| DNA | endonucleolytic activity against apurinic and apyrimidinic sites and a dose-dependent response to DNA that has been X-irradiated, UV-irradiated or treated with OsO4 | Escherichia coli | fragments of DNA | - |
? | |
| DNA | cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site | Escherichia coli | fragments of DNA | - |
? | |
| additional information | enzyme also has DNA N-glycosylase activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 12000-14000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| X-ray endonuclease III | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity against OsO4-treated DNA | Escherichia coli |
| 7.5 | - |
sharp optimum, activity against apurinic and apyrimidinic sites | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.1 | pH 6.2 and pH 8.1: 50% of activity maximum, activity against OsO4-treated DNA | Escherichia coli |
| 7.2 | 7.8 | pH 7.2 and pH 7.8: 50% of activity maximal, activity against apyrimidinic and apurinic sites | Escherichia coli |
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