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Literature summary for 4.2.99.18 extracted from
- Purification and properties of the major apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin (1980), Eur. J. Biochem., 110, 379-385.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | inhibition above 100 mM, 5fold inhibition at 500 mM | Rattus norvegicus |  |
| additional information | inhibition at high ionic strength, factor 5 at 500 mM | Rattus norvegicus | |
| NaCl | inhibition above 100 mM, 5fold inhibition at 500 mM | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromatin | - |
Rattus norvegicus | 785 | - |
| nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | slight stimulation at 10 to 30 mM, inhibition above 100 mM | Rattus norvegicus | |
| Mg2+ | Mg2+ or Mn2+ required | Rattus norvegicus | |
| Mn2+ | Mn2+ or Mg2+ required | Rattus norvegicus | |
| NaCl | slight stimulation at 10 to 30 mM, inhibition above 100 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Rattus norvegicus | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Rattus norvegicus | fragments of DNA | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life: 9 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7 and pH 9: 50% of maximum activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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