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Literature summary for 4.2.3.8 extracted from
- Overexpression, single-step purification, and site-directed mutagenetic analysis of casbene synthase (1998), Arch. Biochem. Biophys., 352, 144-152.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned, expressed and overproduced in Escherichia coli | Ricinus communis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C519W | site-directed mutagenesis | Ricinus communis |
| H131Q | site-directed mutagenesis | Ricinus communis |
| H171Q | site-directed mutagenesis | Ricinus communis |
| H294Q | site-directed mutagenesis | Ricinus communis |
| Q355E | site-directed mutagenesis | Ricinus communis |
| Q356E | site-directed mutagenesis | Ricinus communis |
| Q359E | site-directed mutagenesis | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, wild-type | Ricinus communis |  |
| 0.0032 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutants Q359E and H131Q | Ricinus communis | |
| 0.0033 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutants H171Q and C519W | Ricinus communis | |
| 0.0034 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant H294Q | Ricinus communis | |
| 0.0125 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant Q356E | Ricinus communis | |
| 0.032 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant Q355E | Ricinus communis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | Ricinus communis | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | casbene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Ricinus communis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.359 | - |
recombinant enzyme | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | - |
Ricinus communis | casbene + diphosphate | - |
? | |
| geranylgeranyl diphosphate | regulation of phytoalexin biosynthesis, enzyme produces the antifungal diterpene casbene, a stress metabolite | Ricinus communis | casbene + diphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant Q355E | Ricinus communis | |
| 0.04 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant Q356E | Ricinus communis | |
| 0.2 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutants Q359E and H294Q | Ricinus communis | |
| 0.21 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutant H131Q | Ricinus communis | |
| 0.23 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, wild-type and mutant C519W | Ricinus communis | |
| 0.24 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C, mutants H171Q | Ricinus communis | |
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