Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate | Escherichia coli | - |
trichodiene + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate | Fusarium sporotrichioides | - |
trichodiene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
BL21 DE3 /pZW03 | - |
Fusarium sporotrichioides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
BL21 DE3/pZW03 | Escherichia coli |
BL21 DE3/pZW03 | Fusarium sporotrichioides |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate | mechanism | Fusarium sporotrichioides | |
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate | stereochemistry, mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.222 | - |
- |
Escherichia coli |
0.222 | - |
- |
Fusarium sporotrichioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7S)-6,7-dihydrofarnesyl diphosphate | - |
Escherichia coli | dihydrofarnesene isomers + (E)-6,7-dihydrofarnesol + (3S,7S)-6,7-dihydronerolidol | - |
? | |
(7S)-6,7-dihydrofarnesyl diphosphate | - |
Fusarium sporotrichioides | dihydrofarnesene isomers + (E)-6,7-dihydrofarnesol + (3S,7S)-6,7-dihydronerolidol | - |
? | |
(E,E)-farnesyl diphosphate | - |
Escherichia coli | trichodiene + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate | - |
Fusarium sporotrichioides | trichodiene + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate | formation of trichodiene involves initial generation of (3R)-nerolidyl diphosphate | Escherichia coli | trichodiene + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate | formation of trichodiene involves initial generation of (3R)-nerolidyl diphosphate | Fusarium sporotrichioides | trichodiene + diphosphate | - |
? | |
additional information | reaction proceeds via isomerization of farnesyl diphosphate to (3R)-nerolidyl diphosphate and further to trichodiene | Escherichia coli | ? | - |
? |