Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | lack of substrate protection against inactivation | Pinus contorta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
geranyl diphosphate | 32°C | Pinus contorta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activates | Pinus contorta | |
Fe2+ | the enzyme requires either Mn2+ or Fe2+ as divalent metal ion cofactor | Pinus contorta | |
K+ | activates | Pinus contorta | |
Mn2+ | the enzyme requires either Mn2+ or Fe2+ as divalent metal ion cofactor, maximal activity at 1 mM MnCl2 | Pinus contorta | |
additional information | no activation by Mg2+, Li+ or Na+ | Pinus contorta | |
NH4+ | activates | Pinus contorta | |
Rb+ | activates | Pinus contorta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Pinus contorta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus contorta | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is extremely sensitive to thiol oxidation. Cyclase activity is completely lost when the partially purified xylem preparation is dialyzed against buffer (pH 7.8) devoid of thiol-protecting reagents (dithiothreitol or 2-mercaptoethanol). Other reducing agents, such as sodium ascorbate (5 mM) or sodium metabisulfite (5 mM), fail to protect the cyclases from inactivation, whereas readdition of 5 mM dithiothreitol to the dialyzed preparations restores 54% of the original activity | Pinus contorta |
Purification (Comment) | Organism |
---|---|
- |
Pinus contorta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
xylem | of 2-year-old Pinus contorta | Pinus contorta | - |
Storage Stability | Organism |
---|---|
0-4°C, partially purified preparation is very stable | Pinus contorta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Pinus contorta | (4S)-beta-phellandrene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, SDS-PAGE | Pinus contorta |
Synonyms | Comment | Organism |
---|---|---|
phellandrene synthase | - |
Pinus contorta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Pinus contorta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.5 | 50% of maximal activity at pH 6.6 and at pH 8.5 | Pinus contorta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pinus contorta | isoelectric focusing | - |
4.75 |