Any feedback?
Literature summary for 4.2.3.5 extracted from
- Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity (2008), FEBS J., 275, 1464-1473.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant proteins are heterologously expressed in Escherichia coli, strain BL21(DE3)RP | Neurospora crassa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S127A | amino acid replacements are performed using the QuikChange site-directed mutagenesis kit | Neurospora crassa |
| S16A | amino acid replacements are performed using the QuikChange site-directed mutagenesis kit | Neurospora crassa |
| S16A/S127A | double-mutant protein | Neurospora crassa |
General Stability
| General Stability | Organism |
|---|---|
| apparent stability of all mutant proteins is comparable to that of the wild-type enzyme | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
5-enolpyruvylshikimate 3-phosphate | wild-type enzyme | Neurospora crassa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-enolpyruvylshikimate 3-phosphate | Neurospora crassa | last enzyme of the shikimate pathway, catalyzes the anti-1,4-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate | chorismate + phosphate | chorismate is a precursor for the biosynthesis of aromatic compounds | r | |
| additional information | Neurospora crassa | the dissociation constant for the S127A mutant protein is similar to that of the wild-type enzyme, whereas the replacement of S16 with alanine results in a slight increase of the dissociation constant | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by two-step chromatographic procedure | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of mutant S127A is 6fold lower than that of the wild-type protein | Neurospora crassa |
| additional information | - |
activity of mutant S16A is 70fold lower than that of the wild-type protein | Neurospora crassa |
| additional information | - |
chorismate synthase has an intrinsic NADPH: FMN oxidoreductase activity that enables the enzyme to generate the reduced FMN cofactor (bifunctionality) | Neurospora crassa |
| additional information | - |
no activity of double-mutant protein S16A/S127A, replacement of both serine residues produces a synergistic effect | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-enolpyruvylshikimate 3-phosphate | last enzyme of the shikimate pathway, catalyzes the anti-1,4-elimination of the 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate 3-phosphate | Neurospora crassa | chorismate + phosphate | chorismate is a precursor for the biosynthesis of aromatic compounds | r | |
| additional information | the dissociation constant for the S127A mutant protein is similar to that of the wild-type enzyme, whereas the replacement of S16 with alanine results in a slight increase of the dissociation constant | Neurospora crassa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate synthase | - |
Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | value of double-mutant S16A/S127A with 5-enolpyruvylshikimate 3-phosphate as substrate is below the detection limit | Neurospora crassa | |
| 0.012 | - |
5-enolpyruvylshikimate 3-phosphate | mutant S16A | Neurospora crassa | |
| 0.14 | - |
5-enolpyruvylshikimate 3-phosphate | mutant S127A | Neurospora crassa | |
| 0.87 | - |
5-enolpyruvylshikimate 3-phosphate | wild-type enzyme | Neurospora crassa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | reduced, the S16A/S127A double-mutant protein showy slightly weaker binding of the cofactor | Neurospora crassa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
